Analysis Of Persistent Organic Pollutants By Fluorescence Immunoassay

Persistent organic pollutants (POPs) is persist in the environment, can beaccumulated through biological food chain, cause serious harm to human health andsurvival of chemicals. With the rapid development of science and technology, moreand more POPs is released into the environment, making it a global environmentalproblem of concern. How fast, sensitive and accurate detection of cosmetics, dailynecessities, food and environment of POPs, has become a new challenge to analyticalchemistry and analysis in environmental monitoring. Monitoring work mainly basedon HPLC, GC-MS analysis of large instrument technology, but the heavy complicatedsample pretreatment (including purification, concentration or derivation, etc.),expensive instruments and experimental environment such as demandingshortcomings make it bad for real-time analysis determination of the bulk samples.Thus, developing methods for quick analysis of POPs is of great significance.Based on above, we developed two works as below in this article:(1) Indirect fluorescence immunoassay for the determination oftris(2,3-dibromopropyl) isocyanurate (TBC): construction of enzyme labeledfluorescence immunosensor for detection of TBC, TBC antibody from rabbits wereused for the first time in our laboratory, immune analysis model based on competitive,with horseradish peroxide (HRP) enzyme mark fluorescence immunoassay sensor forTBC selective detection is constructed, TBC can be detected in the detection limit of6.2nM. The proposed method has good selectivity, high sensitivity and accuracy,which with satisfactory recovery rate on the determination of actual samples.(2) Proposed an immunosensor using rhodamine B labeled-octachlorostyrene(OCS) through fluorescence resonance energy transfer for sensitive detection of OCS:OCS antibody were obtained for the first time, based on an indirectly competitiveimmunoreaction, the rhodamine B labeled-OCS competed with OCS in sample forspecific immunoreaction with the limited amount antibodies, followed by mixing withCdTe QDs formation fluorescence resonance energy transfer, giving a determinationlimit of3.8nM. Compared with the traditional method, the proposed immunoassay,low cost, convenience, and high sensitivity was obtained, it has a good applicationprospect in actual samples.