Analysis Method Research And Contamination Levels Survey On Perfluoroalkyl Substances In Alcohol

Perfluorochemicals(PFCs) were industrial compounds, but also new POPs.Perfluoroalkyl substances(PFASs) were the most extensively studied, these compoundshave a unique hydrophobic and oleophobic properties and were widely used. In recentyears, there were PFASs pollution in the global environment, the human body, all kinds offood and food contact materials, and analytical methods on PFASs were not the same in avariety of matrix. In this study, analysis method of16common PFASs in Wine, Chinesespirit and Beer matrix were researched and established, and preliminary investigation ofthe contamination levels of PFASs was studied. The main content and results were asfollows:(1) Analysis method of PFASs in WineWAX weak anion solid phase extraction column was used during the enrichment andpurification on PFASs in Wine, examined the effect of the WAX column on differentcarbon chain lengths PFASs’ recoveries, and optimized the eluent and it’s concentration,and established PFASs in wine with liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) analysis method. The results showed that: WAX column had the strongestadsorption capacity of PFASs; when wine sample’s pH at4.0, the recovery of PFASs weremore conducive to improve;10mmol/L triethylamine-methanol was the best eluent. Thismethod was comfirmed with good reproducibility, high sensitivity and wide linear range,the detection limits of the method on16PFASs were between0.02and0.19μg/L, thequantification limits of in the range of0.08to0.63μg/L.(2)Analysis method of PFASs in Chinese spiritUsing PSA sorbent powder dispersive solid phase extraction during enrichment andpurification on PFASs in Chinese spirit, examined the effect of three kinds of powderadsorbent-C18, PSA and graphite carbon black(GCB) on PFASs’ recoveries, and optimizedoptimized the eluent and it’s concentration sample pH before adsorption, adsorption time,and established PFASs in Chinese spirit with HPLC-MS/MS analysis method. Studiesshowed that:50mg PSA had the best adsorption capacity on PFASs;200mmol/Ltriethylamine-methanol was the strongest eluent; when sample’s pH at7.0, the recovery ofPFASs were prior to adsorption on PFASs. This method was comfirmed with goodreproducibility, high sensitivity and wide linear range, the detection limits of the method on16PFASs were between0.03-0.15μg/L, the limits of quantification in the range of0.10-0.45μg/L.(3)Analysis method of PFASs in BeerA dispersive solid phase extraction in enrichment and purification on PFASs in Beer,examined the effects of three organic solvents-methanol, ethanol and acetonitrile-onPFASs’ recovery and optimized the volume of the organic solvent, the mass of PSA andGCB, a HPLC-MS/MS analysis method of PFASs in beer was established. Experimentalresults showed that: Methanol was added a solution of1:1before PSA adsorption helped toimprove PFASs recoveries; with combination of PSA and GCB adsorption, when PSA at200mg and GCB at20mg, the highest average recovery of PFASs was reached. Thismethod was comfirmed with good reproducibility, high sensitivity and wide linear range,the detection limits range of the method on16PFASs was0.02-0.08μg/L, the range of thelimits of quantification was0.06-0.26μg/L.(4) PFASs contamination levels survey in wine, Chinese spirit and beerThe analysis method of wine, Chinese spirit and beer were used on determination ofPFASs’ concentration in115wine samples,25Chinese spirit samples and30beer samples,the result showed that: Detectable concentration ranges of PFBA and PFBS were0.42to311μg/L and0.20to6.8μg/L in wine; detectable concentration ranges of PFBA, PFPeAand PFOA were in the range of0.25-16.00μg/L,0.34-0.87μg/L and0.20-0.36μg/L inChinese spirit, PFBA, PFPeA and PFOA detectable concentration were in the range of0.12-9.43μg/L,0.08-1.22μg/L and0.26-0.44μg/L.