Study And Evaluation On The Analysis Methods Of Aflatoxins In Food

Aflatoxin is one of the most toxic compounds in nature, and generally exists in cereals, nuts, vegetable oils, dairy products and other daily food, and there is a serious threat to human health. In view of the fact that at present there are a lot of aflatoxin standard methods, but they were not complete and unified. Based on this premise, the aflatoxin detection methods were improved and compared to establish an unified national standard. The full text was divided into four chapters.The first chapter, the nature, the harm and the domestic and foreign limite on aflatoxin were introduced briefly, and the common aflatoxin detection methods in the domestic and foreign were discussed in detail.The second chapter, liquid chromatography and liquid chromatography-tandem mass spectrometry for the aflatoxin Bi,B2,Gi and G2had been improved. Through the optimization of the enzyme linked immunoaffinity column, the extraction solution and flow velocity of derivative liquid and other experimental conditions, the detection methods had the higher sensitivity and accurate detection results. Evaluate4kinds of commonly used ELISA kits, by comparing the limit of quantification, and the recovery of different samples, it showed that to the different samples, the appropriate ELISA kit should be choosed, and before using the ELISA kit, the quality should be tested. On this basis, combined with the other aflatoxin detection method-on-line solid phase extraction-liquid chromatography, compare and evaluate the4kinds of methods. The results showed that, the accuracy of ELISA was lower and not suitable for preliminary screening, liquid chromatography-tandem mass spectrometry method is the best for confirmation, liquid chromatography is better quantitative method for routine sample, online solid phase extraction-liquid chromatography spectrometry would become popular afterwards.The third chapter, detection methods of aflatoxin M1were evaluated. Use2kinds of test strip on the market to detect the different types of milk. The result showed, strip method is a good rapid screening method, but there are more errors. In addition, the milk quality control samples and the milk purchased on the market were detected. The results were consistent.The forth chapter, refer to the methodology of the EU96/23/EC, based on the verification of the standard curve, limit of quantification, specific, accuracy and precision, a relatively quality control plan was established.