Microbial Community Dive Rsity Of Bioaerosols In Qingdao And The Yellow Sea

To understand the microbial community diversity of bioaerosols in Qingdao andthe Yellow Sea, seasonal variation of microbial community in bioaerosols in Qingdaowere analyzed, and the effects of fog and haze on microbial community compositionwere also studied. In addition, the biological aerosol samples in the Yellow Sea werecollected. Comparede the microbial community in bioaerosols in Qingdao with thosesover the sea, the preliminary understanding of the impact of ocean on microbialcommunity was obtained. Samples were analyzed using polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE).The results showed that the bioaerosols in Qingdao has a high microbialcommunity’s diversity, and the microbial communities remained stable during aperiod of similar environmental conditions. There were some differences in microbialcommunities among samples collected at different sampling times and sites. For thesamples collected at the same time, there was a high similarity in microbialcommunity between the coarse and fine particles, but the population abundancedifference was observed between fine and coarse particles.The microbial community in bioaerosols in Qingdao had a seasonal distribution,but under similar environmental conditions, the diurnal variation of the microbialcommunity was not obviously. The diversity of microbial communities in bioaerosolspresented a seasonal variation of autumn> spring> summer> winter in Qingdao. In thestudy area, the correlation between microbial community in bioaerosols andatmospheric temperature appeared positive significant (P<0.05), but no significantcorrelation between microbial community and wind speed or wind direction.The NCBI BLAST analysis indicated that the bacterial community in bioaerosolswas mainly composed of Proteobacteria、Firmicutes、Actinobacteria、Bacteroidetesand Cyanobacteria. In addition, it was also had some sequences which cannot classified and identified in the data base. Most of bacteria sequences were closelyaffiliated with uncultured bacteria. And Proteobacteria and Firmicutes were thedominant species in bioaerosols.To study the bacterial community in bioaerosols during fog and haze days,samples were collected under the the fog and haze days in the Qingdao coastal regionin Januaryand March2013. Compared with the sunny day, we founad that microbialcommunityhad a great change and in fog and haze days. The bacterial compositionbecame more complex in the fog and haze day and the different dominant specieswere also found. Microbial communities in fog days have highly similarity with hazedays. In addition, the composition of microbial community in different months isdifferent, while there was a high similarity between the coarse and fine particlescollected at the same time. Compared with the sunny day, the composition ofbioaerosol community fog and haze weather had larger changes in the fine particlesthan in coarse one. In the fog and haze weather, Bacilli, Pseudomonas,andAcinetobacter class were the dominant species.The study on microbial communities of bioaerosols in different sea showed thatthe microbial communities in the terrestrial bioaerosol samples were more complexthan those over the ocean. There was a high similarity between the coarse particle andfine particle for the same samples. However, there were some common bacterialspecies among the marine and the terrestrial bioaerosols. The dominant species ofterrestrial bioaerosols samples were more than the those in marine bioaerosolssamples. The bacterial community diversity was lower and the number of dominantpopulations reduced more when samples collected in further region from the coast.The microbial communities in bioaerosol were not only affected by the surroundingenvironment, but also affected by the temperature, relative humidity, wind speed andother environmental factors.