Screening Strains Of S. Cerevisiae With The Co-symbiosis Of L. Plantarum IMAU10120for Probiotic Properties

Saccharomyces cerevisiae had already been applied commercially as edible starter clutures, it had achieved industrialization by mixing with Lactobacillus plantarum as complex starter clutures. In this study,112strains of Saccharomyces cerevisiae isolated from fermented milk(goat、horse、camel、cattle and congee) in Mongolia、Inner Mongolia、 Xinjiang、Qinhai and Tibet were screened in vitro for selecting strains of Saccharomyces cerevisiae with the co-symbiosis of Lactobacillus plantarum IMAU10120and testing with simulated gastric fluid, transshipment of gastric and intestinal fluid, bile tolerance and antagonistic property, developing Saccharomyces cerevisiae with excellent probiotic properties.The result showed that30strains of co-symbiosis were screened out(the viable counts and the value of OD600were higher than control group);17gastric juice-tolerant strains were screened out by simulated gastric fluid test(the survival rates of viable counts at pH1.5,2.0and2.5were respectively above30%,60%and80%); through transporting from pH2.5simulated gastric fluid to pH8.0simulated gastrointestinal fluid and bile salt tolerance test, four favorable strains (Saccharomyces cerevisiae IMAU6Y047, IMAU7Y006, IMAU7Y007and IMAU7Y036) could be tolerated the bile salts at the concentration of2.0%environment. IMAU7Y007was considered as bile salt-resistent strain, while IMAU6Y047, MAU7Y036and IMAU7Y006were bile salt-tolerant strains.The zymotic fluid and experimental strains of four Saccharomyces cerevisiae were resistance to three target pathogens, the inhibitory area of IMAU7Y006, IMAU7Y007and IMAU7Y036were significantly higher than the IMAU6Y047. Considering all these, Saccharomyces cerevisiae IMAU6Y047, IMAU7Y006, IMAU7Y007and IMAU7Y036can be used for probiotic potential for further research.