Study On Preparation And Application Of Soy Protein-polysaccharide Polyelectrolyte Microsphere

By using soy protein and natural polymer chitosan and carrageenan polysaccharide as raw material, we adopted the chemical cross-linking method and the complex coacervation method to prepare microspheres, respectively. According to the technical requirements of Radix zanthoxyli coated particles, we studied the production rate and the average particle size of the particles within the scope of0.05mm to0.1mm in diameter. The objectives were to find out the optimum technological conditions to support medicine on microspheres and to verify its feasibility by determining the supporting effects.Firstly, Soy protein-chitosan microspheres with soy protein and chitosan as raw materials were prepared by adopting the chemical cross-linking method. Optical microscopy was used to analyse the particle size distribution. Moreover, the optimum technological conditions were confirmed by using the orthogonal experimental design and single factor experiment. The characterization of the soy protein-chitosan microspheres was carried out by means of electron microscope, infrared spectroscopy, and X-ray diffraction.Secondly, Soy protein-carrageenan microspheres with soy protein and carrageenan as raw materials were prepared by adopting complex coacervation method. The particle size distribution, the optimum technological conditions and the characterization of the soy protein-carrageenan microspheres were carried out by the same means of that of the soy protein-chitosan microspheres.Finally, the alcoholic extract of Radix zanthoxyli was supported on the soy protein-chitosan microspheres and the soy protein-carrageenan microspheres by embedding method, respectively. Then, we should make reasonable analysis of drug loadings, encapsulation efficiency and amount of drug release.The results showed that the average particle size was0.065mm with66.7%of the micdrospheres being in the range of0.05-0.1mm under the condition that the mass fraction of chitosan was2.0%and liquid paraffin (V=100mL) was regarded as continuous phase, methylbenzene (V=40mL) as emulgator, distilled wateras distilled water, glutaraldehyde(V=4mL) as cross-linking agent, respectively. The effects the microspheres prepared by using the chemical cross-linking method would be the best under the above condition with the stirring speed of500r/min. What’s more, the results also indicated that The effects the microspheres prepared by using the electrostatic agglomeration method would be the best under the condition that the concentration of KCl was2%and soy protein content was equal to carrageenan’s with the stirring speed of500r/min. Under this condition,52.4%microspheres reached the requirements and the average particle size was0.084mm. The orange soy protein-chitosan microspheres were tend to relatively stable, while the white soy protein-carrageenan microspheres were tend to have a variety of crystal transformation. We support the same medicine on these to microspheres, however, the results were quite different. Drug loading, encapsulation efficiency and amount of drug release (within24hours) of the soy protein-chitosan microspheres were16.23%,38.29%,42.06%, respectively. Drug loading, encapsulation efficiency and amount of drug release (within24hours) of the soy protein-carrageenan microspheres were23.15%,52.34%,48.23%, respectively.