Preparation And Properties Of High Performance Biomass Carbon-based Protein Imprinted Microspheres

In the field of biotechnology and life sciences,many researches are devoted to the delelopment of protein drugs,medical diagnostics,and the pathogenesis of major diseases based on proteomics.In the above studies,the purification,separation and detection of proteins are particularly important.As a new recognition materials,the protein imprinted polymer has been widely concerned in the filed of protein separation.However,compared with the imprinted polymers based on small molecules as templates,due to the large molecular volume and complex surface structure of template proteins,the imprinted polymers are difficult to meet the requirements of practical applications in terms of recognition,separation volume and separation rate.Therefore,how to construct a high performance imprinted polymer is the key problem in the development of molecular imprinting technology.In this paper,the high performance protein imprinted material was used as the research target,aiming at the difficulty of the template protein with huge volume and complex surface structure.This paper adopts the strategy of combining surface imprinting technology and template immobilization,innovatively utilizes new biomass carbon-based materials as carriers,and through the ingenious design and combination of functional monomer groups to prepare highly recognizable,large separation and fast separation rate of imprinting material.The main research contents are as follows:(1)The biological carbonaceous microspheres rich in carboxyl functional groups on the surface were used as protein immobilization carriers,and dopamine and zwitterionic polymer chains(PVSP)were used as functional monomers units,through surface imprinting technology combined with template immobilization strategy,lysozyme imprinted microspheres with a core-shell structure were prepared.By tuning and controlling various factors such as the content of carboxyl groups on the surface of CFCs matrix,the thickness of the polydopamine imprinted layer and the dosage of PVSP,the prepared CFC-PVSP@MIPs showed the high IF value(3.10),favorable adsorption capacity(68.1 mg g-1)and fast adsorption equilibrium time(120 min).In addition,the imprinted polymer can be separated from binary protein mixtures,which has the potential of practical application.(2)Considering the limited specific surface area of carbon microspheres may result in the relatively low protein immobilization capacity.In order to further increase the adsorption capacity of the MIPs,we first chose the UIO-66 as the carrier of the protein imprinting,and the methyl methacrylamide and the zwitterionic monomer DMAPS as functional units,through surface imprinting technology combined with template immobilization strategy,the protein imprinted material was successfully prepared.The UIO-66@MIPs shown excellent identification(IF=3.2),high adsorption(Qmax=334 mg g-1)and rapid adsorption rate(80 min)of cytochrome c.The adsorption machine indicated that the UIO-66@MIPs recognition of the cytochrome c by the surface of the material.(3)Considering the advantages and disadvantages of carbon nanoparticles and UIO-66,in this chapter,raspberry-like MOF/carbon microsphere composites were prepared by in-situ growth method,and used it as an immobilized carrier for the construction of high-performance protein imprinting carrier material(CN@UIO-66@MIP).In addition,in order to reduce the non-specific binding of the imprinted material to the protein,the zwitterionic monomer DMAPs was used for the synthesis of the protein imprinted layer.The results showed that the application of MOF/carbon microsphere composite carrier could greatly increase the protein retention capacity.Moreover,the recognition performance of imprinted materials could also be optimized by adjusting the dosage of DMAPS.Moreover,the adsorption experimental results showed that the MIPs had high adsorption capacity of(815 mg g-1),the rapid adsorption balance time(40 min)and the excellent recognition ability(IF?6.1).Finally,The development of this material will lay a research foundation for further application in the fields of protein extraction,isolation,sensing,immunodetection and drug controlled release,which is conducive to promoting the application and development of protein imprinting technology.