Study On Process Of Ultrasonic Extraction Polyphenol、 Polysaccharide From Rose Residue

Rose residue is by-product which contains rich nutrients in the process of roseextract essential oil. At present, there is no a set of effective treatment method fordealing with them. Improving rose residue comprehensive utilization value not onlyconducive to the development of rose industry rapidly and sustainably, but alsoprotect the environment and also has great economic and social benefits.Taking rose residue as raw material, the extraction and purification of roseflavonoids as well as extraction of rose polysaccharide by ultrasonic assistedextraction technology were studied in this paper.Concentration of ethanol,ultrasonic power,ultrasonic temperature,ultrasonic time,extraction times and material-liquid ratio were mainly considered to show the effecton the extraction rate of roses polyphenols. Based on this experiment, theBox-Benhnken method was adopted to analyze the response surface methodologyexperiments. The results showed that the optimal conditions for the extraction ofroses polyphenols as follows: concentration of ethanol70%,ultrasonic power62.5w,ultrasonic temperature63℃, ultrasonic time16min, extraction time3, material-liquid ratio1:62(g:mL).Under the optimal conditions, the extraction ratio of rosespolyphenols was2.732mg/g by using ultrasonic-assisted extraction technology. TheIR spectrum showed that there are many active functional groups in rose polyphenolssuch as hydroxyl,carbonyl etc.Ultrasonic assisted extraction technology was used to extract rosepolysaccharides. The single factor experiment and orthogonal experiment were usedto research the effect of material-liquid ratio, ultrasonic power, ultrasonic temperature,ultrasonic time and extraction times, concentration of ethanol on the extraction rate ofrose polysaccharides. The results showed that the order of the factors whichinfluenced on rose polysaccharides extraction ratio was concentration ofethanol>ultrasonic temperature>material-liquid ratio>ultrasonic power. The optimumconditions were as follow: concentration of ethanol70%, ultrasonic temperature20℃,material-liquid ratio1:35, ultrasonic power125W, extraction times3.In this optimal conditions, the extraction ratio of roses polysaccharides was4.126mg/g. Rosepolysaccharide contains pyran ring and furan ring.Rose polyphenols were purified by ethyl acetate which was taken as extractionsolvent. Factors which had influence on extraction rate of rose polyphenols weremainly considered, including extraction time, stewing time and extraction times. Theresults showed that the best purification conditions were extraction time30min,stewing time40min and extraction times3.Under the conditions, the purity of rosepolyphenols was64.38%.