Investigation On Near-infrared Light Triggered Controlled Release Nano-drug Delivery System Of Magnetism Carbon Nanotubes

In this study， a near-infrared light triggered sustained and controlled releasedrug delivery system was constructed. This system was comprised of carbonnanotubes-iron oxide hybrid nanocomposite (CNTs-IONP) loaded with Docetaxel(DTX) and covered with glycerylmonostearate (GMS). While the system wasirradiated by808nm laser, DTX can be released due to the disruption of the thermalsensitive GMS with the heat produced by CNTs-IONP. Study on this project will beconducted including formulation optimization, drug release mechanism investigationon the developed delivery system in vitro and in vivo, near-infrared light triggeredcontrolled-release, distribution and its anti-cancer effect in vitro and in vivo.In order to optimize the prescription of the CNTs-IONP-DTX-GMS, the rate ofreactants, concentration of surfactant, power and frequency of the ultrasound wereinvestigated by the one-factor experimental design. The controlled release drugdelivery system CNTs-IONP-DTX-GMS was characterized by dynamic laserscattering (DLS) and transmission electron microscopy (TEM) so on, which indicatedexcellent loading capacity of DTX for this system. It also shows excellent stability insaline, PBS, cell culture medium and fetal bovine serum (FBS). The particle size ofthis system is193.5±3.78nm, ζ potential is-25.04±0.61mV, which performedfavourable.The drug sustained-release ability can be valuated in vitro. The result indicatedthat compared with microspheres and DTX, CNTs-IONP-DTX-GMS exhibitedobviously sustained and controlled release effect.With the mice bearing S180sarcoma, the concentration and distribution ofCNTs-IONP-DTX-GMS in different organs and tumor tissue were measured byHPLC. It is shown that tumor, live and lung can be selectively targeted byCNTs-IONP-DTX-GMS. Meanwhile, the in vivo anti-cancer effect ofCNTs-IONP-DTX-GMS was evaluated with DTX as control. The results indicatedthat CNTs-IONP-DTX-GMS performed better therapeutical effect and andlessirradiation, the results show that the heat efficiency of CNTs-IONP has strong toxicity compared with Docetaxel.This study also revealed the heat efficiency of CNTs-IONP under808nm laserirradiation with concentration and time dependency. Besides, DTX released from thedrug delivery system can be significantly increased when irradiated by808nm laserfor2min due to the disruption of GMS by the CNTs-IONP produced heat. Moreover,the in vivo experiment on S180bearing mouse model indicated that CNTs-IONP hasstrong hyperthermia therapy effect. In addition, while the system was applied withDTX synergetically, the cancer cells can be killed more efficient with hyperthermiaand chemotherapy.