The Synthesis Of Magnetic Mesoporous Silica Nanoparticles And Its Application In Biomedicine

In recent years, magnetic mesoporous silica nanoparticles (MMSN) have an extensive research on dignose and therapy. MMSN have mechanical, heat and chemical stability, which lead them possess extensive biomedical application prospect. MMSN also possess high surface area, pore volume, narrow tunable pore distripution, easy modifiable surface and magnetism target, which make them have an adventange on drug delivery system.Angiogenesis is important for the therapy of various vascular diseases, wound healing, and tissue regeneration. The angiogenesis process is controlled by different growth factors, and among them, bFGF is one of the most widely studied growth factors. However, bFGF is very susceptible to enzymatic and thermal degradation in vivo, when it was administrated injectively, resulting in the short half-life. Heparin is a highly sulfated and polyanionic glycosaminoglycan which can bind affinities to different growth factors, enables bFGF to bind with its receptors and to be preserved from proteolytic degradation.Magnetic mesoporous silica nanoparticles were synthesised throughing a one pot synthesis. Sphere-like magnetite aggregates were successfully gained as cores of the final materials via assembling in the existence of PVP30and CTAB. The material has a superparamagnetism property with a saturation magnetization value of20.3emu g-1. Moreover, the combination of heparin and fluorescence-labeled MMSNs exihibit the result particles (denoted as MFMSNs-HP) with fluorescence properties and magnetic, good dispersity in the PBS solutions and cell culture medium, the anticoagulant activity in the blood stream, and the controlled release of basic fibroblast growth factor (bFGF). Furthermore, the cell viability assays of bFGF demonstrate that MFMSNs-HP is nearly no toxicity to human umbilical vein endothelial cells (HUVEC) up to the concentration of200μg mL-1, and the proliferation activity of bFGF binded with MFMSNs-HP could be presevered at least6days. We also load DOX on MFMSNs-HP and find DOX can also be sustained, and DOX-loaded MFMSNs-HP can kill HepG2cell. All of these indicate that MFMSNs-HP may serve as a multifunctional carrier.