The Research Of The Integrative Aeration Biological Filter Of Phosphorus And Nitrogen Removal For Efficiency Of Processing Sewage And Microbial Population Structure

By using the molecular ecology technology as means of research, with homemademagnetic filter material and ordinary ceramsite filter material, this paper studies the microbialspecies, quantity and space change rule under different working conditions. By combining withremoval efficiency of the reactor, this paper discusses the microbial communities play a role inprocessing sewage by aeration biological filter.At first, this paper builds and domesticates the integrative aeration biological filter ofphosphorus and nitrogen removal. Then from two aspects of chemistry and microorganism,this paper studies the hanging membrane characteristics of the reactor, analyzes its microbialpopulation structure. The results shows that, the total hanging membrane phase needs to32days to complete, COD removal in18days can reach70%, the ammonia nitrogen in20dayscan reach70%, which shows that the aerobic heterotrophic bacteria’s value-added rate isfaster than aerobic autotrophic bacteria’s. In water of the reactor, the microbial community isrich, the dominant population is relatively similar, mainly for Verrucomicrobia,Gemmatimonadete and Proteobacteria.Then this paper studies two reactors’ efficiency of phosphorus and nitrogen removal andthe microbial community’s succession and interaction under different conditions:First is to change the ammonia nitrogen load, and run74days. The results shows that,with the ammonia nitrogen load increasing, two reactors’ CODcr removal rate is in slowdecline, the average CODcr removal rate in three phases is71.55%,69.59%,66.59%and71.55%,69.94%,66.58%respectively; removal of ammonia nitrogen is hit by differentdegree of impact in the early, but No.2is better than No.1reactor to recover; the effect oftotal phosphorus removal isn’t affected by large, the average removal rate is27.53%and27.41%respectively. Using the means of molecular ecology to analyze, the microbialcommunity species is richness, superiority strains are obvious, and belong to seven groups,respectively is Nitrospirae, Verrucomicrobia, Proteobacteria, Firmicutes, Cyanobacteria,Chlamydiae and Acidobacteria. With the ammonia nitrogen load increasing, the superioritystrains of water decrease, but the number of each type is in gradually increasing; each stage has its own superiority strains, shows that the microbial population has a succession ofchange, some superiority trains will be replaced by another ones; their unique superioritytrains exist in the two reactors, shows that two different filter material will be suitable for thesurvival of different microbial population. In three stages, diversity and evenness of samplesis the lowest and the dominance of samples is the highest in the first stage, which shows thatthe diversity of microbial system improves, the relationship formed between species is morecomplex, and the impact resistant ability of ecological system is stronger.The second is to change the total phosphorus load, and run100days. The results showsthat, with the total phosphorus load increasing, before dosing coagulant, two reactors’CODcr removal rate isn’t affected by large, precipitation’s average removal rate is17.41%and17.94%respectively, filter’s average removal rate is60.79%and62.81%respectively;after dosing coagulant, precipitation’s average removal rate is35.81%and37.9%respectively, which is about twice as high compared with no dosing coagulant; filter’saverage removal rate is53.77%and47.55%respectively, compared with no dosingcoagulant, removal rate reduces. Before dosing coagulant, precipitation’s ammonia nitrogenremoval isn’t affected by large and is very limited, which is3.70%and4.16%respectively;filter’s average removal rate is89.11%and89.53%respectively, the ammonia nitrogenremoval has no obvious change; after dosing coagulant, precipitation’s average removal rateis6.63%and6.01%respectively, which still under10%; filter’s average removal rate is90.37%and90.77%respectively; compared with no dosing coagulant, removal isn’t affectedby large, which shows that coagulant dosing to remove ammonia nitrogen has little effect.Before dosing coagulant, effect of two reactors for removal of total phosphorus is not ideal,removal rate reduces slowly, precipitation’s average removal rate is7.04%and6.49%respectively; filter’s average removal rate is15.59%and19.96%respectively; after dosingcoagulant, precipitation’s average removal rate is51.26%and52.44%respectively;compared with no dosing coagulant, removal rate increases largely, and with the totalphosphorus load increasing, the total phosphorus removal rate is higher; filter’s averageremoval rate is23.31%and24.77%respectively, compared with no dosing coagulant,removal rate has no obvious change, which shows that chemical phosphorus removal play amajor role. Using the means of molecular ecology to analyze, the microbial community species is richness, superiority strains are obvious. There are plenty of microbial strains existin the experimental stage, and some unique strains for individual stages. Before dosingcoagulant, the superiority strains belong to four groups, respectively is Verrucomicrobia,Acidobacteria, Proteobacteria and Chlamydiael; after dosing coagulant, the superioritystrains belong to seven groups, respectively is Verrucomicrobia, Chlamydiae, Proteobacteria,Chloroflexi, Nitrospirae, Acidobacteria and Cyanobacteria.