Study On New Systems Of Chemiluminescence And Its Application In Pharmaceutical Analysis

Chemiluminescence(CL) detection is a new, trace, high-speed analyticaltechniques, which has been successfully applied in pharmaceutical analysis,bioanalysis, immunoassay fields. Development of new systems and combination withother techniques expend its further applications, also laid the foundation for rapiddevelopment. This paper focuses on the forming of new CL systems by carbonquantum dots and ultra-oxidation state complexes, then successfully used for sampleanalysis. The main content includes the following sections:(1) The fluorescent carbon dots (CDs) of good biocompatibility and low toxicityprepared by a carbonation method, it can significantly enhance the CL intensity ofluminol-potassium ferricyanide. It was found that the CL intensity of CDs-luminol-potassium ferricyanide was strongly inhibited in the presence of2-ME.Fluorescent carbon dots-enhanced CL method for the determination of2-ME wasdeveloped. Under the optimum conditions, the method with a detection limit of4.1×10-10g/mL was successfully applied for determination of2-ME in human serumand pharmaceutical preparations. Different from other metal quantum dots,fluorescent carbon dots of good biocompatibility and low toxicity shows broadapplication prospects.(2) It was found that a chemiluminescence signal can produced by DPA directoxidation of mitoxantrone. Based on the phenomenon, a new sensitive CL methodwas established for analysis of mitoxantrone combining with flow injectiontechnology. The directly oxidation chemiluminescence involving single agent caneffectively avoid interference with other reagents, which has advantages of goodreproducibility, high sensitivity and good selectivity. Under the optimum conditions,the concentration of mitoxantrone showed a good linear relationship with the relativechemiluminescence intensity and the detection limit is1.9×10-10g/mL. It wassatisfactory for the application to determine mitoxantrone in pharmaceuticalpreparations and human serum sample, the mechanism was discussed by combinedwith UV absorption spectra and fluorescence spectra. (3) A CL signal was observed between luminol and diperiodatoargentate(Ⅲ) inalkaline medium. After the addition of gimeracil into the system, the CL intensitycould be greatly enhanced. A rapid and sensitive flow-injection CL method was firstlydeveloped for the determination of gimeracil, factors affecting the CL intensity wereinvestigated and the possible mechanism was studied.(4) The luminol-potassium ferricyanide system can produce a certain intensity ofsignal in an alkaline medium. It was found that ketoconazole can significantlyenhanced the intensity of the system, based on this phenomenon a new method for thedetermination of ketoconazole was established. Under the optimum conditions, therelative CL intensity of the system was linear with the concentration of ketoconazoleand the detection limit of was3.1×10-9g/mL. The proposed method was applied tothe determination of ketoconazole tablets and biological samples, the possible CLmechanism was discussed briefly.