Study On Biology Recycle On Waste Rubber With Microbe

Millions of waste rubber materials are discarded every year. The huge amount of waste rubber is an environmental problem of great concern. The regeneration of waste rubber emerges as the times require.The research uses three techniques with two strains to recycle the waste rubber, focusing on microbial desulphurization. One way is to add crumb rubber to the bioreactor which contains S.cerevisiae already, and then the rubber material is devulcanized as the metabolization of micro-organism. The second way is to cultivate and gather the mass of strain first, mix them with crumb rubber and then the biodesulphurization will take place under the fixed condition. The third way is to blend crumb rubber with Thiobacillus ferrooxidans (T.f) by enrichment and screen to desulfurize.Based on the process of glutathione and rubber, we have discovered that the surface desulphurization efficiency of natural rubber and styrene butadiene rubber with glutathione was33.65%,40.96%respectively, while45.45%with the co-culture of yeast and nature rubber. GSH yield reached378.17mg/L after36hours cultivation of S.cerevisiae R1in5L fermentor. Then gather the yeast strain to extract GSH by cell lysis Effect of yeast extract on microbial recycle of waste natural rubber was investigated by the swell ratio of rubber. The optimum conditions for the recycle process were obtained as follows. The amount of solvent and phase-transfer catalyst was1.85mol and2.75×10-2mol/100g rubber powder, respectively. The amount of yeast sludge was equal to add1.0g of GSH. The reaction was carried out at50℃for4h. The swell ratio is1.49times with the yeast extract up to now.There were three bacterial strains of degrading sulfide isolated from contaminated sulfide soil. The isolated was identified as T.f and was designated strain W-Y-1, W-Y-2, W-Y-3respectively. The results of experiments indicated that nephelometery and the iron oxidation ratio was better with the condition:culture temperature30℃, initial pH3.0, and initial ferrous sulfate concentration8%.The co-culture of T.f and waste rubber has been studied. Sulfur removal ratio of NR and SBR under the condition of30℃,150rpm on20days with the strain W-Y-1which is not purified enough was59.90%,32.07%. While the surface desulphurization efficiency of natural rubber with the purified strain W-Y-1,W-Y-2and W-Y-3was69.17%,66.87%,65.80%.