Research The Process Of Extracting The Resveratrol By Enzymatic Hydrolysis From Rhizoma Polygoni Cuspidate

The research of production process about resveratrol extracting from the naturalplant rhizoma polygoni cuspidate is one of the significant subjects in biologicalpharmaceutics research and development. The resveratrol exists in the form of glucosidein the rhizoma polygoni cuspidate. There are something problem in the practicalextracting process such as unsatisfying enzymatic hydrolysis effect, the lower extractionratio of resveratrol, the long production cycle, etc. For overcoming these weakness anddifficulties occurring in the production process, this work is typically focused on theresearch of the resveratrol extraction process from the rhizoma polygoni cuspidate. Ithas also carried out more systematic research like the detection and separation of theresveratrol from the crude extraction.The research has established a quantitative determination for determining thecontent of polydatin and resveratrol by HPLC, which is carried out by Agilent EclipseXDB-C18reversed phase column and gradient elution with mobile phase ofacetonitrile-water. Then the standard working curves of resveratrol and polydatin aremade by external reference method. The fitting result of resveratrol’s standard curve isA8.779821061m131944.0952，r210.9943. The fitting result of polydatin’s standardcurve isA4.56441062m217264.0476，r220.9986. The linear fitting degree of thedetermination is high and the result is precise and satisfying. Finally the content ofresveratrol and polydatin in the natural plant rhizoma polygoni cuspidate are determinedby HPLC, which the resveratrol’s mass basis content is0.444%and the polydatin’s is2.076%.The process of extracting the resveratrol from rhizoma polygoni cuspidate iscarried out by direct enzymatic hydrolysis before methanol extraction. After selectingthe significant influence factors by single factor test, the response surface analysis isconducted and the optimal extracting conditions of rhizoma polygoni cuspidate’senzymatic hydrolysis is found that the optimal rhizoma polygoni cuspidate’s particlesize is200mesh; the optimal ratio of solid and liquid is1:20(g:ml); the optimalenzymatic hydrolysis temperature is42℃; the optimal pH of the NaAc-HAc buffersolution is nearly4.72; the optimal enzyme consumption is1:500(g enzyme: g rawmaterial); the optimal stirring and warm-keeping reaction time is22h; the solid residueis repeatedly extracted4times,10minutes per time, by the methanol which the usage is 1:10(g:ml) per time. The significant factors which decide resveratrol’s extraction ratioorder like that B. enzymatic hydrolysis temperature> A. the enzyme consumption> C.the pH of enzymatic hydrolysis, which the enzymatic hydrolysis temperature has themost apparent influence on the module. The extraction rate of resveratrol is1.389%andthe conversion rate of polydatin is97.03%, which are calculated on the dry basis ofrhizoma polygoni cuspidate.After screening the macroporous resin, the NKA-9macroporous resin is selectedas the best separating medium for resveratrol, and the absorption and desorptionbehavior of rhizoma polygoni cuspidate extractive on the NKA-9macroporous resinextractive have been researched as well as the operation conditions are optimized. Afterprocessing by the macroporous resin, then the elution is purified by Al2O3column anddecolorized by activated carbon, finally crystalized by freeze drying and made the highpurity resveratrol production. The determination proves that the mass purity of theresveratrol is98.32%and the purification recovery rate is92.61%...