Study On Extraction Of Active Ingredients From Caulis Polygoni Multiflori And Their Weight Loss And Lipid-lowering Activity

With the improvement of modern living standards,obesity has become a serious disease that is harmful to human health,and the treatment of obesity is also a major problem.Compared with western medicine,Chinese herbal medicine is favored by people because of its wide range of sources,low side effects and low dependence.Caulis Polygoni Multiflori(PMC)which is one of the Homologous Chinese herbal medicines that can be used for health foods production allowed by the Ministry of Health has effects on anti-oxidation,blood lipid reduction and sleep quality improvement.It can meet the requirements of the people in need of the current society.At present,there are few reports on its activity and mechanism both here and abroad.The in-depth and systematic research on PMC will be conducive to develop health products with different effects.In this experiments,the anti-oxidation and lipid-lowering activities of active fractions from PMC were studied.Five active fractions were extracted from PMC,include 75% ethanol extracts(PMC-Z),chloroform extracts(CHL),ethyl acetate extracts(EA),n-butanol extracts(n-Bu)and water phase residues(W).Fours main compounds,stilbene glycoside,apigenin,emodin and physcion,were identified by HPLC method.Except CHL,the other four active fractions played good effects on anti-oxidation in vitro,among which n-Bu showed the best antioxidant effect,followed by EA.Study on active fractions from PMC effect in the proliferation of 3T3-L1 preadipocytes and its lipid accumulation during adipocyte differentiation.The results showed that the active fractions could inhibit the proliferation and differentiation of 3T3-L1 preadipocytes,among which the effects of n-Bu was the best,followed by EA.EA and n-Bu were selected to study their effects on fat accumulation in Caenorhabditis elegans(C.elegans).The results showed that both EA and n-Bu could inhibit the lipid accumulation and triglyceride(TG)production.At medium and high concentrations,both them increased the activity of superoxide dismutase(SOD),catalase(CAT)and decrease the content of malonaldehyde(MDA)which showed good antioxidant effect in vivo.EA and n-Bu can significantly inhibit the expression of sbp-1,fat-7 and fat-2 genes.The difference between them is that EA can significantly inhibit the expression of mdt-15 gene while n-Bucan significantly down-regulate the expression of nhr-80 gene.Study on the obese C57BL/6J mice(C57 mice)induced by high fat diet showed that EA and n-Bu significantly inhibited the growth of weight and abdominal fat of mice,and the effects are not by inhibiting calorie intake,and the high dose of n-Bu(200 mg/Kg.d)achieved the best effect.n-Bu significantly inhibited the increase of total cholesterol(TCHO)and high-density lipoprotein(HDL-C)in mice induced by high-fat diet in a dose-dependent manner.EA showed no significantly in the inhibition of TCHO but had significant difference in the inhibitory effect of HDL-C.Both EA and n-Bu had certain inhibitory effect on serum leptin and lipopolysaccharide(LPS)increased by obesity,and increased the activity of SOD and CAT but reduced MDA contents in liver to some extent,which the best effect was in the high dose group of n-Bu(200 mg/Kg.d).In conclusion,both EA and n-Bu showed good antioxidant activity and lipid-lowering activity in vivo and in vitro.According to the results of component analysis,it was inferred that the pharmacological activity of EA might be related to the content of stilbene glycoside,and the pharmacological activity of n-Bu might be related to the content of emodin.