Fabrication Of Antibacterial Electrospun Nanofibrous Composites Fibers Containing Ciprofloxacin Hydrochloride As A Wound Dressing

Nanofibers produced by electrospinning technique have many advantages such assmall diameter, high surface to volume ratio, highly porous structure of nanofibers,absorbability, hemostatic properties, semi permeable for respiration of cells, and abetter conformation to the wound surface. For these advantages, electrospinningtechnique was used for the fabrication of poly (vinyl alcohol)(PVA)/regenerated silkfibroin (SF) composite nanofibers and poly (vinyl alcohol)(PVA)/Sodiumcarboxymethylcellulose (NaCMC) composite nanofibers, loaded with ciprofloxacinHCL (CipHCL) as a wound dressing.Firstly, Electrospun PVA/SF/CipHCL composite nanofibers were stabilized againstdissolving in water by heating in oven at155℃for5min. Incorporation of CipHCLinto electrospun nanofibers was confirmed by SEM and FT-IR spectra. Further themechanical properties test illustrated that the addition of CipHCL enhanced themechanical properties of PVA and PVA/SF nanofibers. The release rate of CipHCLfrom mats was modulated by incorporation of PVA with SF. Meanwhile the heattreatment resulted in the reduced drug release rate. The antibacterial activities againstEscherichia coli (E. coli)(Gram negative) and Staphylococcus aureus (S. aureus)(Gram positive) organisms were evaluated by disk diffusion method; and results suggested that electrospun PVA/CipHCL and PVA/SF/CipHCL composite nanofibersshowed a remarkable antibacterial activity. Furthermore, the biocompatibility ofas-prepared electrospun PVA/SF/CipHCL composite nanofibers was investigated bycell viability study. It was found that the composite nanofibers promoted the cellproliferation and attachment with low cell cytotoxicity, so the as-prepared electrospunPVA/SF/CipHCL composite nanofibers can be regarded as safe drug delivery carrierfor wound dressing applications.Secondly, electrospun PVA/NaCMC/CipHCL composite nanofiber,PVA(shell)/CipHCL(core), PVA(shell)/NaCMC&CipHCL andPVA&NaCMC(shell)/CipHCL(core) core-shell nanofibers were stabilized againstdissolving in water by soaking in ethanol solution containing1%CaCl2for one hourto exchange Na+ions in NaCMC by Ca+, after that heating in oven at155oC for5min is applied. The treated nanofibers were relatively highly stable after treatment.Incorporation of CipHCL into electrospun nanofibers was confirmed by SEM, TEMand FT-IR spectra. Further the mechanical properties test illustrated that the additionof CipHCL enhanced the mechanical properties of PVA and PVA/NaCMC nanofibers.TEM results showed the formation of core/shell structure of nanofibers in case ofPVA(shell)/CipHCL(core), PVA(shell)/NaCMC&CipHCL(core) andPVA&NaCMC(shell)/CipHCL(core) electrospun nanofibers. It is observed that, invitro drug release of treated PVA/NaCMC composite nanofibers, loaded with CipHCLprovides a sustained and controlled release pattern of CipHCL over48hours andcore/shell structures nanofibers showed more slow release of CipHCL as compared to blending electrospinning nanofibers. The antibacterial activities against Escherichiacoli (E. coli)(Gram negative) and Staphylococcus aureus (S. aureus)(Gram positive)organisms were evaluated by disk diffusion method; and results suggested thatelectrospun PVA/CipHCL and PVA/NaCMC/CipHCL composite nanofibers showed aremarkable antibacterial activity. Furthermore, the biocompatibility of as-preparedelectrospun PVA/NaCMC/CipHCL composite nanofibers was investigated by cellviability study. It was found that the composite nanofibers promoted the cellproliferation and attachment with low cell cytotoxicity, so the as-preparedelectrospun PVA/NaCMC/CipHCL composite nanofibers can be regarded as safe drugdelivery carrier for wound dressing applications.