The Effect Of Biochemical Regulation Factor On Ring Cleavage Of Phenol By Pseudomonas Stutzeri N2

Phenol and other phenolic compounds were important industrial raw material, andthey were widely used in coking, paper, dyes, pesticides, phenolic resins andpetrochemicals. Thus, phenolic compounds were kind of common and highly toxicpollution in the environment. Microbial degradation of phenolic pollution was acost-effective way. Although many strains which were used for degradating phenol hadbeen isolated, phenol mineralization and harmless were always unresolved. Therefore,the study of the conditions for phenol mineralization by the efficient strains topurification of phenolic pollutants has practical significance.In this paper, based on preliminary findings, the metabolism of carbon and oxygenwere two angles ways to study a high concentration of phenol resistant Pseudomonasstutzeri N2, and phenol mineralization and harmless’s characteristics. Study thefollowing conclusions:(1) Methyl alcohol, ethyl alcohol and acetone which were co-existence carbonsource could accelerate ring cleavage of catechol when N2stains degraded phenol, andethyl alcohol was more conducive to N2stains degraded phenol via ortho-pathway andmeta-pathway. When phenol was the sole carbon source, mebolites were catechol andp-hydroxy benzoic acid which were not ring-opening products. When phenol and ethylalcohol were the sole carbon source, intermediates added to cis, cis-muconic acid,2-HMS and2-hydroxy-2-muconic acid which were ring-opening products.(2) When ethyl alcohol and phenol coexisted, under aeration head standingoxygenation, oscillation aerobic shaker and non-oxygenated oscillating shaker threeconditions, the amount of dissolved oxygen in the growth medium were approximatelyat about6.5-7.2,4.5-6.6and2-4.6mg·L-1. Catechol1,2-dioxygenase and catechol2, 3-dioxygenase existed N2bacterial cells simultaneously. Under the conditions ofoscillation aerobic shaker and non-oxygenated oscillating shaker, catechol1,2-dioxygenase which existed N2bacterial cells that had grown11hours, its activity0.136,0.116μmol. mg (protein)-1. min-1. The more abundant oxygen, the two catecholdioxygenase activity existed N2bacterial cells is higher.(3) Under aeration head standing oxygenation, oscillation aerobic shaker andnon-oxygenated oscillating shaker three conditions, TOC which was from thePseudomonas stutzeri N2metabolic process of phenol showed rapid decline. Under thecondition of aeration head standing oxygenation conditions, there are60.89%of phenoldegradation has been mineralized during72h. Plus ethyl alcohol and increasing thevolume of oxygen in the reaction system were conducive to N2bacteria on phenolmineralization and harmless.The results of this study lay the foundation for Pseudomonas stutzeri N2applications in complex environments.