Isolation, Purifition And Optimization The Fermentation Conditions Of The Polysaccharide Of Alginate And Inulin Degradation Bacteria

As the study on Brown Algae Oligosaccharides, Fructooligosaccharide(FOS),Fructose goes deeply, people have gradually known and accepted their physiologicalactivity. Means of producing Brown Algae Oligosaccharides, Fructooligosaccharide(FOS), Fructose become a Research Focuses. enzymatic with light reaction, thereaction was easy to control, more safe and less by-products etc, While its advantageshave been Various Report. So Screening of degradation bacteria which can produceDegradation enzymes, have caught the focus of peoples’ research.this study is to obtainbacteria witch can produce inulinase and other bacteria witch can produce alginate lyase.A strain H3which could effectively producing Alginate lyase was isolated from marineenvironment and identified, A dominant bacterium JY30which can effectively degradeexo-inulinase was isolated from soil and identified.Using sodium alginate as the sole carbon sourc during the experiment. A strain H3which could effectively producing Alginate lyase was isolated from the rot disease part ofrot laminaria japonica. A preliminary observation on the morphology of cyclina this strain.Study on the species identification by the application of16S rDNA, derived is that its16Sribosomal DNA compared with Halomonas approximately90%similarity. At the sametime, using self-made inulin as the sole carbon source. A strain JY30which couldeffectively producing inulinase was isolated from rhizosphere with jerusalem artichoke.Analysis of inulin degradation product by high performance liquid chromatography(HPLC). The degradation product after the reaction between strain JY30and the substrate is detected mainly fructose. Further results is that produced inulinase major displaysexonuclease activity. In order to determine the optimum medium composition of thatstrains. By single factor experiment to determine inulin as the optimum carbon, theoptimum concentration of60g/L; optimum nitrogen source is yeast extract powder, theoptimum concentration of70g/L; pH=7.0. The central composite design CentralComposite Design and response surface analysis were adopted to optimize the threenotable factors. While establishing and analyzes the influencing factors and reducingsugar concentration of the reaction product with The mathematical models. The resultShow: its equation of the coefficients of determination (R2) of fitting curve were97.66%.Explanation that the fitting effect was excellent. Optimization on medium composition forinulin9.37%, yeast extract7.3%, pH7.01. In this process parameters, reducing sugarconcentration of enzyme hydrolyzation substances is0.026mg/mL. Compared with theprevious unoptimized, reducing sugar concentration of enzyme hydrolyzation substancesincreases from0.026mg/mL to0.25mg/mL, Improved9.5.