Studies On The Method For Simultaneous Detection Of Drugs Residues In Aquatic Product

In recent years, the phenomenon that the abused and illegal use of antibiotics, disinfectants, dyestuffs and other drugs in the process of breeding have been increasingly serious with the expansion of the scale of the aquatic agriculture. Drug residues of aquatic products for the quality and safety of aquiculture has become one of the major risk factors in our country. The drugs, such as malachite green, crystal violet, chloramphenicol, sulfa, quinolone, macrolides, are common to be easily abused or illegally used in the process of aquatic products breeding, even for the unequivocally prohibited drugs (malachite green and chloramphenicol). The corresponding standard methods of drugs above for residue detection have been formulated in our country. However, the lack variety of residual quantity detection in these methods results in the low detection efficiency. These methods have been unable to figure out the increasingly stringent of emergency disposal, day-to-day supervision of food safety and enterprise self-control detection. Therefore, developing a method that simultaneously drugs residue detection can provide a new technical basis for effective monitoring of drug residues in aquatic products and self-inspection-and-self-control of enterprises in China.In this study, the aquatic products, such as Pseudosciaena crocea, are used as samples. The pretreatment methods, which contain extraction, purification and concentration, are utilized and liquid chromatography-mass spectrometry is performed for the detection. Finally, the detection methods are established to simultaneously detect malachite green, crystal violet, chloramphenicol, sulfa, quinolone, and macrolides of six categories of all49kinds of drugs in the aquatic products. All the drugs have a good linear relationship in the corresponding concentration range and the correlation coefficients are more than0.99. The recovery of fortified samples in different concentration levels of range from57.6%to100.5%. Relative standard deviation area is between1.6%and10.0%。This method greatly shortens the time of sample pretreatment; meets the requirement of relevant standards for the detection limits of items under test and is applicable to simultaneously rapid detect several drugs.