| In view of the problems of current optical biosensing technology, such aslow selectivity, high cost, complex operation, in recent years, many researchersfocus on the development of the simple operation, low cost, portable opticalbiological detection technology. Because the discs have the advantages of lowcost, good optical properties, this novel disc-based detection technology isexpected to become a new generation of biological sensing technolog. In thispaper, we proposed a DVD-based technology, which utilizes optical disc storagetechnology based on discs, combines micro-nano machining andnanotechnology, and skillfully use commercial electronic equipment-opticaldrive/CD player. Based on this technology, this paper has realized a highsensitive, rapid quantitative detection of multiple samples by digitally readingthe error signal. In addition, this paper also develops a new type of colorimetrybiosensor, achieves the visual and simultaneous detection of multiple samples bythe colorimetric analysis. The following are the main research contents andconclusions:Heavy metal pollutions seriously affect the people’s health, cause incalculable damage to the ecological environment, so the rapid detection ofinexpensive and sensitive detection of heavy metals cause people increasingattention. In this paper, a sensitive and cost-effective method for thesimultaneous quantitation of trace amounts of Hg2+and Pb2+in real-worldsamples has been developed using DNA molecular beacon probes bound to bonafide digital video discs (DVDs). With specially designed T-rich or G-rich loopsequences, the detection is based on the strong T-Hg2+-T coordination chemistryof Hg2+and the formation of G-quadruplexes induced by Pb2+, respectively. Thismethod exhibits a wide response range and low detection limits for both Hg2+and Pb2+. This method compares favorably with other optical sensors for theon-site testing.On-site oral fluid testing for drugs of abuse has become prominent fortaking immediate administrative action in an enforcement process. Herein, thispaper develops a DVD technology-based indirect competitive immunoassayplatform for the quantitative detection of drugs of abuse. A microfluidicapproach is adapted to prepare multiplex immunoassays on a standard DVD-R,an unmodified multimode DVD/Blu-Ray is utilized to read signal, and a freedisc-quality analysis software program is employed to process the data. TheDVD assay platform is successfully demonstrated for the simultaneous,quantitative detection of drug candidates (morphine and cocaine) in oral andblood fluids with high selectivity. This method exhibits a wider detection rangeand smaller detection limit comparable with that of ELISA methods. Therefore, as a new type of portable biosensor, this method has a great application prospectfor realizing the on-site detection of drugs.A novel immunochip is explored to simultaneously and colorimetricallydetect of morphine, cocaine and amphetamine in body fluids. The proposedmethod is based on the production of specific multiple colour after reaction on aPolycarbonate (PC) base. Under optimized conditions, there is a detection rangeof1-1500,1-900and1-900ng/mL to amphetamine, morphine and cocainerespectively. The limit of the developed method is determined to be0.01,0.01and0.05ng/mL for morphine, cocaine and amphetamine respectively, with thefunction of tyramine signal amplification (TAS). So this colorimetric sensor hasa great development space for realizing the rapid, on-site and low-cost drugdetection.Above all, this novel DVD-based technology has the potential to become anew generation biosensor, and has a board application prospect for on-sitechemical analysis and medical diagnostic. |
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