| As a kind of pharmaceuticals and personal care products (PPCPs), antibiotics have been increasingly concerned for their ubiquity in the environment and their health effects. Recently, scientists pay more attention to the antibiotics’threat to ecosystem. However, researchers often focus on few antibiotics because absent of analysis method. So we developed a fast detection method for33antibiotics residues, including quinolones (QLs), sulfanilamides (SAs) and tetracyclines (TCs) in coastal waters, sediment and organisim.The method consists of solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, using electrospray ionization (ESI) in positive mode. The SPE was performed by Oasis HLB and Oasis MCX cartridges. Chromatographic separation on a C18column was achieved using a gradient eluent containing methanol and water both with0.1%formic acid. Typical recoveries of the analytes ranged from58.2to109.3%in water samples, from56.9to85.4%in sediment and60.8to86.8%in organism. The precision of the methods, relative standard deviations (RSD) were below14.6%,12.5%and13.2%, respectively. The limits of detection (LODs) varied from0.45to8.06pg.The developed method was applied for the determination of target analytes in samples from Bo Sea and Yellow Sea.. The results showed the method was validated to analysis antibiotics in water, sediment and organism. The main contaminants around Bo Sea and Yellow Sea were QLs, which were generally detected in all of the samples and the total concentration levels were up to μg/L. Compared with the other regions, the concentrations of QLs were much higher, while the levels other antibiotics were similar with the developed regions. The northwest part of Bo Sea, Jinzhou Bay and Laizhou Bay were polluted most severely. Antibiotics were seldom detected in sediment, but the were universality existed in organism. The main residues were QLs and TCs. SMR in same organic samples were higher than the limit concentration. |
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