Studies On Behaviors Of Reverse Micelles Systems Extraction Of Soy Protein Process

With the wide applications of reverse micelles in extraction of protein, preparation ofpeptides and amino acids, drug synthesis, nano-materials preparation, etc, the nature ofreverse micelles system getting more and more attention. Especially soy protein have wideapplications in the food and other industries while reverse micelle extraction is a new type ofhigh efficient separation technology, which can separate plant protein and oil at the same time.Therefore, it is of universal significance to study the micellization behavior of.reversemicelles during the extraction of soybean proteins.This paper determined a applicable method of determining the critical micelleconcentration (CMC) of reverse micelle system formed by Di-2-(ethyl hexyl) sodiumsuccinate (AOT). Then, analyzed the factors of reverse micelles CMC, the formation ofreverse micelles thermodynamic and its kinetic behaviors of extraction of soy protein at theCMC; studied the influence of concentration of surfactant for reverse micelles particle sizeand examined the various factors on the conductivity of reverse, Last but not least,exploredthe optimum conditions of extracting soybean protein by reverse micelle system, comparedthe structure and properties of soy protein which extracted by different reverse micellesystems and extraction ways.Compared four methods, including surface tension, UV spectrophotometry,fluorescence spectrophotometry (two fluorescent probes), which can determinate the CMC ofAOT. Selecting coumarin C343as a fluorescent probe was wise, for its advantages of highsensitivity and selectivity, less interference. The research of CMC showed that: temperature,ionic strength buffer solution and added co-solvent increase the difficult of the formation ofreverse micelles, while long straight-chain organic solvent conducive to the formation ofreverse micelles.Thermodynamic behavior of reverse micelle showed that: surfactant can spontaneouslyform reverse micelles, the rise of temperature is not conducive to micellization phaseseparation process. Formation of reverse micelles is a process of surfactant molecules from the clutter of a single state to orderly arrangement forming reverse micelle phase, the drivingforce of the formation of reverse micelles is entropy-driven results; The kinetics studies of theextraction of soybean protein by reverse micelles at its CMC indicated that: this processingcan use the "un-reacted core model" to Simulate, soy protein break free from other substancesof FFSBM,"inner diffusion ’role, is the main control speed steps.Composite reverse micelle was shown to be feasible when used the extraction process ofsoy protein. Infrared spectra of the secondary structure of soy protein extracted by reversemicelle system showed that: reverse micelles had an impact on the secondary structure of soyprotein: the percentage of α-helix reduced, β-sheet and disordered structures hundred ofcontent increased, the content of β-corner also declined; SDS-PAGE electrophoresis showedthat: reverse micelle system extracted soy protein subunit molecular weight is smaller,inadequate ability of extract larger protein molecular weight, non-ionic surfactants Tween60can increase the size of reverse micelles, in favor of extracted the larger protein subunitmolecular weight. Soy protein fluorescence spectra showed that: the position of soy proteinfluorescent emission peak blue shift, reverse micelles extraction of soy protein in the structurewill be more stretch, the internal group will be exposed.