The Anti-Oxidant Activity Of The Methanol Extract Of Tsao-Ko

In order to provide theoretical basis for developing new food resource, the methanol extract were abstracted from tsao-ko produced in Yunnan, and its anti-oxidant activity in vivo and in vitro, the mechanism of action, and the condition of high-performance liquid chromatography (HPLC) were investigated.The anti-oxidant activity of the methanol extract of tsao-ko in vitro showed the DPPH and ABTS clearance of methanol extract of tsao-ko which greater than rutin and curcumin when the concentration is0.14mg/mL with0.3ml. The methanol extract of tsao-ko showed favorable anti-oxidant ability, just had lower FRAP value than rutin and curcumin, showed stability as natural antioxidant.Aged mice were induced by D-galactose combined with high fat diet. To study the anti-oxidant effect of methanol extract of tsao-ko to aged mice. Compared with the control group about the data of liver, the activity of SOD and GSH-Px in the aged mice conducted with tsao-ko was significantly improved (p<0.01), the content of GSH in the aged mice conducted with high dose of tsao-ko was significantly improved (p<0.01), the content of MDA in the aged mice conducted with tsao-ko was lower (p<0.05). Compared with the control group about the data of plasma, the activity of SOD of mice conducted with low dose of tsao-ko was improved (p<0.05) and significantly improved with high dose (p<0.01), the activity of GSH-Px of mice conducted with high doses of tsao-ko was significantly improved (p<0.01), the content of8-ISO-PGF2a in the aged mice conduced with high doses of tsao-ko was lower (p<0.05), the content of GSH of mice deposed with tsao-ko was significantly improved (p<0.01), the content of MDA in the aged mice conducted with tsao-ko was significantly lower (p<0.01)The best chromatographic conditions of the methanol extract of tsao-ko were that using WondaSil-C18as stationary phase, acetonitrile and water (1:7) as mobile phase, adjusting pH with2%acetic acid. The velocity was0.3mL/min and the absorption peak was detected in279nm. Accordanced with HPLC conditions, identified its molecular weight by HPLC-MS/MS, determined the category of active ingredients to provide the basis for the separation and purification of each monomer.