Jerusalem Artichoke Extract Polysaccharides, Separation, Purification And Analysis

Jerusalem artichoke is a D-fructose polysaccharides by β (1â†'2) glucosidebond connected together, It is a very safe food additive that the human body hassignificant health functions, Jerusalem artichoke requirements for low growthenvironment in recent years, Jerusalem artichoke by pay attention to people at homeand abroad, So there must be a good prospect.In this paper, the use of Jerusalem artichoke tubers as raw material extraction ofpolysaccharides of Jerusalem, purification, bleaching, purity identification, physicaland chemical properties. Where the hot water extraction method comparison andultrasonic extraction method of polysaccharide, and then determine the optimalconditions for protein except lime method, the use of activated carbon fordecolorization. The results are as follows:(1) Polysaccharide jerusalem artichoke were extracted by hot water extraction andultrasonic extraction. First by single factor experiment, merit each condition, and thento explore the use of orthogonal experiment. The optimum conditions in which hotwater extraction studies obtained: extraction time60min, temperature90oC,solid-liquid ratio1:18, feed alcohol ratio of1:3, extracted3times. The optimumconditions of ultrasonic extraction: extraction time40min, the solid-liquid ratio1:20,temperature80oC.(2) Using lime method, Sevag method, TCA method, hydrochloric acid method forthe extraction of inulin were deproteinized, including lime method by single factorexperiment showed that protein clearance lime highest law.(3) The use of activated charcoal (powder) of Jerusalem was purified polysaccharidebleaching treatment, first by single factor experiment, merit each condition, and thenusing the orthogonal experiment to determine the optimal decolorization conditions:2%of the amount of activated carbon, bleaching time20min, bleaching temperature60oC, the decolorization rate can reach88.9%.（4）According to the phenol-sulfuric acid method to measure the total sugar refining Jerusalem artichoke polysaccharide content:98.4%, reducing sugar contentof3.2%, the polysaccharide content=total sugar-sugar content, so the content ofpolysaccharides is:95.2%. colorimetric method based on measuring the inulincoomassie brilliant blue tight for protein content:0.26%.（5）By UV scanning to determine the purified polysaccharide sample does notcontain impurities such as proteins and nucleic acids, a preliminary analysis of theinfrared detector structure of polysaccharides, including polysaccharides varioussignificant functional groups. By scanning electron microscopy and electronmicroscopy can be seen, the physical characteristics of inulin.（6）The use of HPLC determination of inulin application monosaccharidecomposition, first we’ll Jerusalem artichoke acid hydrolysis of polysaccharides intosimple sugars and then into HPLC testing, the results show that the Jerusalemartichoke is a polysaccharide composed of glucose and fructose are twomonosaccharide composition, and D-fructose is first showing, D-fructose content ofwhich more than glucose.