Preparation And Application Of A Molecularly Imprinted Material Of10-Hydroxy-2-Decenoic Acid

10-hydroxy-2-decanoic acid (10-HDA), also known as queen acid, is a significant unsaturated fatty acid in royal jelly. Prior studies indicated that10-HDA showed variety of physiological functions with enhanced immunity, anti-bacterial, anti-cancer, anti-tumor, treatment chemical injury. Because some of the physiological activity of royal jelly have a great relationship with10-HDA, so the content of10-HDA is identified as an important factor that affect royal jelly quality. GB9697-2008clearly states, the conent of10-HDA in qualified royal jelly must be less than1.4%, and it must be greater than1.8%in superior product. Currently there a variety of royal jelly and its products on the market, therefore some fake and shoddy products appeared. It is necessary to establish a sensitivity and high selective analytical technique with efficient sample pretreatment for determination of10-HDA.In this paper, with10-HDA as the template,4-vinyl pyridine as the functional monomer, and N,N’-methylenebisacrylamide as the cross-linker, a novel10-HDA molecularly imprinted polymer with good selectivity and sensitivity was prepared by bulk polymerization method. With a series of optimization experiments, the optimum conditions of synthesis for this imprinted polymer were found. And this imprinted polymer was evaluated through adsorption experiments, and characterized by Fourier transform infrared and electron microscopy. The results of adsorption experiments illustrated that the imprinted polymer had fast mass transfer rate (the adsorption equilibrium was achieved in20min) and high saturated adsorption capacity (Qmax=858.4mg g-1) for the template.A determination method for10-HDA in royal jelly was developed with the prepared polymer as a selective enrichment sorbent in a solid phase extraction coupled with high-performance liquid chromatography. Besides in this research the parameters affecting the procedure including the pH of loading solvent, loading flow rate, leacheate, eluent and the amout of eluent were optimized. Under the optimum conditions, the detection limit (S/N=3) was0.94μg L-1.The linear plots with r2>0.99were achieved over a range of10-1000μg L-1and the peak area precision (RSD) for nine parallel determinations was below4.54%.At last the royal jelly, royal jelly lyophilized powder and royal jelly oral liquid were spiked with three levels of10-HDA (royal jelly were final spiked10g kg-1,20g kg-1,40g kg-1, royal jelly lyophilized powder were final spiked25g kg-1,50g kg-1,100g kg-1,royal jelly oral liquid were final spiked12.5mg L-1,25mg L-1,50mg L-1)and analyzed after simple pretreatment. Good recoveries of these samples were achieved ranging from86.53%-103.28%, which proved this new method was usefulness applied to the detection of10-HDA.