Study On Preparation And Properties Of Hemoglobin Molecularly Imprinted Polymer

The separation and purification of protein molecules is one focus of proteomics research content. Due to the variety and difference of physical and chemical properties for proteins, it is very hard for their separation and purification. Molecularly imprinted polymer with convenient operation, designability, cheap price and specificity for template recognition has opened up a new way. Ionic liquid is a green and non-toxic solvent with good biocompatibility for protein. In this research, a new molecularly imprinted polymer was prepared to absorb hemoglobin by combining the properties of ionic liquids and molecular imprinting technique.In the experiment, molecular imprinted polymer was prepared by the method of bulk polymerization using phosphate buffer solution (pH7.0) as reaction system, hemoglobin as template molecule, acrylamide and ViProIm+SO3-as functional monomer, MBA as crosslinking agent, APS and TEMED as initiator. The amount of the reaction solvent, ViProIm+SO3-addition and the type of ionic liquid were optimized. The structure of ionic liquid, the morphology and structure of the polymer were characterized by FT-IR and H1-NMR. The properties of polymer including adsorption kinetics, adsorption equilibrium, adsorption thermodynamics and the ability and stability of special recognition on Hb were also evaluated.From the obtained results, it was found that the imprinting factor a achieved to2.01when using ViProIm+SO3-as functional monomer, reaction solvent is7mL and the molar ratio of ViBuIm+Cl-/AAm was1/10. Increasing the pH of adsorption solution, the adsorption capacity of polymer on Hb and imprinting factor increased and then decreased. The results of adsorption kinetics and equilibrium experiment showed that Hb-MIP has fast adsorption rate and adsorption capacity reached up to82%at about2h. And twelve hours later, the adsorption process achieved equilibrium. According to Langmuir adsorption model, the Qmax value was calculated to be176mg g-1. By using BSA, Lyz, Cyt c as competition protein, the selective ability of Hb-MIP was evaluated. These results showed that the Hb-MIP not only has high adsorption capacity, high imprinted factor and good selectivity.