Study On A Rapid Determination Method On The Basis Of Estrone Molecular Imprinted Polymer

A novel molecularly imprinted polymer has been prepared using estrone as template, acrylamide (AM) as functional monomer, ethylene dimethacrylate (EDMA) as cross-linker at the ratios of1:3:6by bulk polymerization molecular imprinting technique. The imprinted polymer was evaluated by static and kinetic adsorption experiments. The results showed that the imprinted sorbent exhibited good recognition, selective ability and offered a faster kinetics for the adsorption and desorption of the template estrone. The results of the Scatchard model evaluation indicated that the adsorption isotherm of imprinted polymer towards the template molecule were in good accordance and with linearity. The saturated adsorption capacity(Qmax) of imprinted sorbent toward estrone was10.88mg g-1.A method was developed with this polymer as enrichment sorbent for molecularly imprinted polymer in situ enrichment of estrone color reaction method to detect trace estrone in environment. The parameters affecting the procedure including the color reagent, the weight of MIPs, loading flow rate and time were optimized in this research. With a loading flow rate of1.0mL min-1for sampling10.0mL of1.00μg L-1estrone-methanol solution, then add in watery KOH-ethanol solution1.0mL and color reagent1.0mL of DNB15.00g L-1reacted1h in dark. The detection limit was10.00μg L-1.To evaluate the usefulness of the novel imprinted sorbent, the samples of lake water and tap water from Tianjin university of science&technology, determined to be free of estrone, were spiked with three levels of estrone (10.00μg L-1,50.00μg L-1and100.00μg L-1) and analyzed after simple pretreatment under optimal experimental conditions. At each concentration, evident change of color in lake water and tap water, respectively. It is proved that the detection of estrone in environment is feasible in this method.