Design, Construction Of Novel Electrochemiluminescence Immunosensor And Its Application In The Determination Of Cancer Markers

Cancer markers mainly refer to the substances origined from the secretion of cancer cells, and they usually exist in the body fluid. Generally, the occurrence or quantitative change of cancer markers in the body fluid can present the nature of the tumours, and the determination can be used to study the occurrence of tumours, cell differentiation and cell function to realize the diagnosis, classification, prognosis and goal-directed therapy. At present, the world health organization (WHO) and health departments all over the world have considered the accurate analysis of cancer markers as the primary task. Though the clinical analyses such as enzyme-linked immunosorbent assay, radioimmunoassay, mass spectrometric immunoassay and fluoroimmunoassay have been widely carried out for the determination of cancer markers in developed economies, they are often not applicable in developing countries and resource-poor countries and regions, because these analytical systems are too expensive, large, complicated, and dependent on specific infrastructures and professional operation personnel. Therefore, it is of considerable interest to the further research for rapid, simple, sensitive and accurate alternative methodology for determination of tumor markers.In this paper, to reliaze the point of care testing, rapid and portable determination of cancer markers in the body fluid, we combine the applications of electrochemiluminescence with microfluidics in the bioanalysis to develop microfluidic electrochemiluminescence immunodevices. According to the construction study of the microfluidic electrochemiluminescence immunodevices, the main research is as follows:1. For low-cost, portable detection of cancer markers, NiMH rechargeable battery (1.20V) was used to take the place of conventional electrochemical workstation to develop a constant-potential electrochemiluminescence (ECL) system, and the principle was investigated. A constant potential-triggerd ECL microfluidic immunodevice was fabricated to detect the cancer markers for the developing countries and resource-poor countries and regions. The principle and properties of the immunodevice was investigated to make the immunodevice commercialized.In this way, we realized the low-cost, fast, portable and accurate determination of the cancer marker in the body fluid.2. To expand the application of constant-potential ECL system, a lithium battery (3.0V) was combined with a self-assemble voltage-controller to produce a voltage-tunable power device with an output voltage of0.01-3.00V. Based on the single-analyte microfluidic paper-based analytical immunodevice, an ECL multiplexed microfluidic immunodevice was designed and fabricated to solve the difficulty of simultaneous determination of multiple cancer markers. In this way, we realized the high-through test of cancer markers.3. To fabricate a low-cost immunodevice, chromatographic paper was used as the substrate to develop ECL microfluidic paper-based analytical immunodevice to detect single or multiple cancer markers, and the principle for the determination was investigated.4. To further expand the types and the fabrication methods of microfluidic immunodevice, indium tin oxide was used as the substrated to construct a three-electrode electroluminescence device. Based on the flow injection electroluminescence system, a solid ECL immunosensor was developed using magnetic materials as the solid support, and the properties of the solid ECL system were investigated.