Label Of Electrochemiluminescence Reagents And Their Application In Biological Analysis

The increasing demands of disease diagnostics and therapeutic analysis require the development of sensitive and accurate detection of disease-related proteins. Developing the methodology of protein tumor marker recognition elements that reveal reproducible improvement and sensitive enhancement is crucial in proteomics and clinical diagnostics. DNA hybridization detection has recently attracted much attention due to its diverse applications including clinical diagnosis, environmental monitoring, and the detection and characterization of viruses, bacteria, and parasites. It is essential to develop precise and convenient DNA sensors at extremely low concentration. The development of practical sensors for the detection of toxic heavy metals has been always a subject of considerable researches. Lead ion (Pb2+) and mercury ion (Hg2+) act as severe environmental pollutants and pose severe risks to humans. They can usually circulate in soil and groundwater to result in an environmental contamination through a variety of natural and anthropogenic sources in plants and animals. Therefore, sensitive and rapid tracking of Pb2+and Hg2+(both in vitro and in vivo) is significant for environmental protection as well as disease prevention and treatment.In our paper, we prepared various nanocomposites for electrochemiluminescence (ECL) labels with amplification techniques. We designed various biosensors for analysis detection, with the advantage of easy operation, wide linear range, high sensitivity and low-cost.According to the application study of ECL labes in biosensing, the main research is as follows:1. A novel Ru-silica(Ru(bpy)32+-doped silica) capped nanoporous gold (NPG)(Ru-silica@NPG) composites as ECL label was used for labeling the second antibody with amplification techniques. A sandwich-type ECL immunosensor was prepared for carcinoembryonic antigen detection on gold nanoparticles (AuNPs) modified glassy carbon electrode.2. A novel ECL reagent named as carbon nanocrystals (CNCs) with low-lost, low cytotoxicity was prepared as well as three dimensional (3D) mesoporous PtAg biometallic nanocomposites. And the newly designed PtAg@CNCs composite was prepared as ECL regent for labeling antibody. An ultrasensitive ECL immunosensor was developed based on carbon nanotubes-chitosan/AuNPs (CNT-CHIT/AuNPs) composite modified screen-printed carbon electrodes for prostate protein antigen detection.3. A novel CNCs as ECL reagent with low-lost and low cytotoxicity was prepared as well as3D mesoporous PdCu biometallic nanocomposites. And the newly designed PdCu@CNCs composite was prepared as ECL regent for labeling DNA strands. An ultrasensitive ECL DNA biosensor was developed based on NPG electrode and PdCu@CNCs composite for DNA detection.4. The CNCs capped silica nanoparticles (Si@CNCs) and Ru(bpy)32+-AuNPs aggregates (Ru@AuNPs) were both used as novel ECL labels in our case with different operational potentials. The functionalized wax-patterned3D paper-based ECL device with advantages of fast, cost-effective, simple, easy to use and dispose of. An ultrasensitive ECL device was developed for Pb2+and Hg2+detection on one paper working zone.