The Electrochemiluminescence Imaging System Is Used For The Detection Of Tumor Markers And The Integrated Research Of Diagnosis And Treatment

Electrochemiluminescence(ECL)imaging is a detection method based on redox reaction,which uses optical instruments such as photomultiplier cameras to convert the electrical signals of electrical biomass into images.At present,ECL imaging has been successfully applied to tumor tissue detection,protein detection,single nanoparticle detection,live cell-matrix detection,tumor marker detection,etc.The integration of diagnosis and treatment is a new method for tumor research.The detection of tumor markers is achieved by outputting a visual signal.After the detection,the targeting of the multifunctional drug probe is used to make the tumor efficiently apoptosis,and to realize the integrated research of tumor diagnosis and treatment.This experiment combines electrochemiluminescence imaging with integrated diagnosis and treatment to build an integrated electrochemiluminescence imaging system for diagnosis and treatment.By outputting ECL images,the detection of tumor markers micro RNA-21 and nucleolin was successfully achieved;the multifunctional drug probes and photothermal targeting tumor markers have successfully achieved efficient treatment of tumors.The specific research work is as follows:(1)Electrochemiluminescence imaging combined with chemical-photothermal therapy for microRNA-21 detection and tumor treatmentMicroRNA-21,as a biomarker for early diagnosis of tumors,participates in the regulation of tumor generation,growth and metastasis and expressed in almost all human malignancies.In this paper,electrochemiluminescence imaging is used for the detection of micro RNA-21.Tumor cells produce over active oxygen under the stimulation of drug probe Au NCs@PMA.Reactive oxygen and luminol emit light under the stimulation of voltage.The light signal was converted into an image by an electron multiplying charge coupled device,and the electrochemiluminescence image of micro RNA-21 was successfully output.After detection,the tumor cells apoptosis under the damage of high concentration of active oxygen;in addition,the nanomaterial Au NCs that construct the drug probe has a good photothermal effect,and can convert the absorbed light energy into heat energy under the excitation of near infrared light.Tumor cells are apoptosis under selective local high temperature;under the combined effect of chemical-photothermal therapy,the purpose of highly effective tumor treatment is achieved.(2)Electrochemiluminescence imaging combined with dual drug therapy for nucleolin detection and tumor treatmentNucleolin plays an important role in regulating cell proliferation,survival and apoptosis,and it can promote the biological function of cells to maintain a steady state,which is of great significance for the study of complex tumor growth processes.In this paper,we used electrochemiluminescence imaging for the detection of nucleolin.Based on the electron transition and reactive oxygen-luminol redox reaction,we successfully output the electrochemiluminescence image of nucleolin in a single tumor cell.After imaging,the synergetic drug probe MSN@PMA@DOX was specifically recognized by the tumor marker nucleolin and released anti-tumor drugs.The doxorubicin(DOX)in the probe induces apoptosis of tumor cells by inhibiting the synthesis of genetic material nucleic acids in cells,and phorbol 12-myristate 13-acetate(PMA)stimulates tumor cells to produce high concentration ROS to destroy DNA and proteins and cause tumor cell apoptosis.Under the synergetic apoptosis drugs,highly effective targeted therapy for tumors is achieved.