Electrochemical Study On Buffer Capacity Of ATP, ADP And AMP

Intracellular pH is an important aspect of the intracellular environment. Many physiological processes occur on the membrane interface, these processes have a very closely relationship of pH change on membrane interface. Changes in intracellular pH can potentially affect virtually all cellular processes, including metabolism, membrane potential, cell growth, movement of substances across the surface membrane, state of polymerization of the cytoskeleton and ability to contract in muscle cells. Changes of pH determine the activity of enzyme and influence the reaction the enzyme catalyzes. The pH can influence the oxidation-reduction potential.ATP is the direct resource of energy for most activities in the organism, Chemiosmotic theory indicates that the pH gradient is the driving force for ATP synthesis in mitochondria. Ca2+, Mg2+and other divalent metallic ion is the high level content in the human body, they can combine with ATP, then change the original structure with the new complex compound, this change can reduce its ability of receiving the proton, also it can promote the hydrolyzation. ATP can hydrolyze to ADP and inorganic Pi.The buffer capacity of inorganic Pi is much more than the ATP. so the change of content for phosphate radical can influence the buffer capacity in the body then form the gradient of pH. this gradient can involve with the processes of biological activities.This paper uses the classical reversible redox couple of hydroquinone and p-benzoquinone to research how can influence the cyclic voltammogram with the solution of ATP、ADP、AMP. We also investigate the change of electrochemistry behaviors due to adding divalent metallic ion to the solution, discuss the factors about the buffer capacity of ATP, summarize the regulation of the electrochemical behavior with different concentration of the solution.The first oxidation peak of hydroquinone has no relationship with its own concentration but relate to the concentration of solution of AT、 ADP、AMP. The current of the first reduction peak of benzoquinone rises up with the increased concentration of ATP、ADP、AMP. Benzoquinone is the proton-acceptor, ATP can release the proton under acidic condition, so the first reduction of benzoquinone is much more obvious than it is under the condition of physiology pH.After ATP bonded with divalent metallic ion or it hydrolyzed to ADP and inorganic Pi, the buffer capacity of solution changes, this can influence the change of pH then have a impact to the organism.