Study On The Ultrasound-assistant Extraction And Antioxi-dation In Vitro Of Blackberry Seed Oil

Blackberry seeds are by-product of the processing of blackberry, which holds 85% of blackberry dregs. The oil content ranges 15% to 20%.At present, the method of extraction of blackberry seed had a low oil yield. The fatty acid components and antioxidant activities in vitro of blackberry seed oil have not been reported. With blackberry seeds as material, the research used microwave-assistant extraction, ordinary temperature merceration extraction, Soxhlet extraction, ultrasound-assistant extraction to extract the blackberry seed oil, and then analyzed its fatty acid compositions and antioxidant activities in vitro.Thc research provided the theory basis with exploitation and utilization of blackberry seed oil in the food industry.The research results were as follows:①The moisture content, crude fat content, crude protein level, ash content, carbohydrate content of blackberry seeds were 11.01%,19.29%,4.85%,1.69%, 63.16%,respectively.②With microwave-assistant extraction, ordinary temperature merceration, Soxhlet extraction, ultrasound-assistant these four methods to extract the blackberry seed oil, the results indicated that the method of ultrasound-assistant was the best way to extract the blackberry seed oil. The optimum parameters were petroleum benzene(30℃-60℃) as extraction solvent, solid to liquid ratio 1:10 (g/mL) ultrasound exacting time 10 min, ultrasound power 400W.The oil yield under the processing optimum condition reached 17.06%.③Gas chromatography-mass spectrometry (GC-MS) was used to analyze the fatty acid components of the seed oil respectively by microwave-assistant extraction, ordinary temperature merceration, Soxhlet extraction, and ultrasound-assistant extraction. The results indicated that the fatty acid components of blackberry seed oil were linoleic acid, linolenic acid, palmitic acid. The fatty acid compositions of blackberry seed oil consisted of more than 90% unsaturated fatty acids. Linoleic acid was the main fatty acid, and it reached more than 76%, and a-linolenic acid reached higher than 15%. ④Compared with other three means, the scavenging rates of blackberry seed oil to DPPH and hydroxyl radicals by ultrasound-assistant extraction were the highest. The blackberry seed oil inhibited erythrocyte hemolysis induced by H2O2, MDA generation and mitochondria swelling induced by Fe2+ and Vc...