Nmr Studies Of Chicken Embryonic Development Process

The T1 -weighted and ^-weighted Magnetic Resonance Imaging (MRI) have been used to study the morphogenesis of chicken embryo and other contains in the egg during the earlier hatching process. T2 values of amniotic fluid, yolk and egg white have been measured by the means of Multi Slice Multi Echo (MSME) sequence.In-vivo 'H Magnetic Resonance Spectroscopy (in-vivo *H MRS) has been used to analyze the ingredients of amniotic fluid, yolk and egg white. The changing tendencies of the relative contents of phosphorous contained substances have been detected by in-vivo 'P Magnetic Resonance Spectroscopy (in-vivo 'P MRS) during the whole hatching process. In-vivo ]P MRS proved the catabolism of adenosine 5'-triphosphate (ATP), phosphorous ester and phosphocreatine (PCr) when the embryo dead. The results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed.The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1-dimention and 2-dimention Nuclear Magnetic Resonance (NMR) methods. This work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. Also, it provided a new way to study the process of embryo developing.