Insect Cells Of Several Orthoptera Taxonomy

In this paper, the chromosomal conventional karyotypes and C-bandingpatterns on seven species of Orthoptera from Songhua Lake and Daqinggou and soon were studied. They belong to five genera of four families. Squashing and BSGmethod were used in the preparation of slides for microscopical examination. Thecells in metaphase of mitoses or meioses were selected for cutting, pasting,analyzing and drawing, and the relational data were analyzed by Excel andMicrosoft SPSS. At the same time, author drew the following several conclusionsby comparing ,analyzing and discussing .1. The chromosomal number of two species of Oedipodidae, namely Celesskalozubovi Adel and Mecostethus baichengensis Sp.nov., and two ones ofCatantopidae,namely Primnoa cavicerca Zhangand P.mandshurica(Rme.), is 2n ( <♂>) =23. Sex determining mechanismsbelong toXO.All the chromosomes are terminal centromere withcenteomereC-banding.However, other cytological characters are differentamonggenera or species and can offer basis for cytotaxonomy. We found obviousdifferences between Primnoa cavicerca Zhang and P. mandshurica(Rme.)by calculating and variance analysis .2. Two species of Arcypteridae, namely Chorthippus brunneus huabeiensisXia et Jin and Ch. minutus Zhang, belong to same genus and have commoncharacters in cytology.The chromosomal number is 2n( <♂>)-17.Euchromosomes consist of metacenteic and telocentric chromosomes. Sexchromosomes are telocentric and sex determining mechanisms belong toXO. Bycomparing we knew the two species have relatively farther relative.3 .Teleogryllus derelictus with 27 chromosomes,which belongs toGryllidae, is distinctly different from Acrididae, but similar to Teleogryllus emma.4. Chorthippus Fieb. and Euchorthippus Tarb. are more related than others inkaryotypes and C-banding patterns. However, they are independent because ofdissimilarities. Therefore, the conclusions are coincident on morphology andcytology.5. Primnoa F.-W., Anapodisma Dov.-Zap., Zubovskia Dov.-Zap. ,SinopodismaChang and Pedopodisma Zheng are genera without flying ability. They aresimilar to each other in cytology. This may be a kind of result that theyacclimatized themselves to similar environmental conditions duringperennial evolution.