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Ascomycetes Ds-9701 Cell Culture Characteristics And Cell Products

Posted on:2006-03-30Degree:MasterType:Thesis
Country:ChinaCandidate:Q B WanFull Text:PDF
GTID:2190360155460116Subject:Cell biology
Abstract/Summary:PDF Full Text Request
We carried out the optional research of the method that be acceptable for breaking up the cell wall of Ascomycetes DS-9701 .and used analysis of SDS-PAGE to research expressional distinction of soluble protein of cell during Ascomycetes DS-9701 was cultivated under different temperatures. Meantime researched cell growing effect of Ascomycetes DS-9701 under a variety of nutrient condition with mothed of direct cross design. We got good result about breaking up the cell wall, the mycelial cell was entirely broken up when the cell of Ascomycetes DS-9701 was disposed by breaking up the cell wall,the leaving numbers of generative cell were 0.132 and 0.178 and 0.115 and 0.239.The analysis of SDS-PAGE show it exists certain distinction that the expression of the soluble protein of cell.there are effects about cell growing character of Ascomycetes DS-9701 under a variety of nutrient condition.we carried out the experiment that optimizes the medium that cultivated cells of Ascomycetes DS-9701 in deep solution for producing the red pigment,got the optimal formulation of medium for producing the red pigment. got curves of culture process and logarithmic growth of thallus about cells of Ascomycetes DS-9701 was cultivated in deep solution for producing red pigment,ascertained optimal cultivating condition about cultivated the strain producing red pigment in deep solution.
Keywords/Search Tags:Ascomycetes DS-9701, solutional deep cultivation of cells, optimize medium, research of breaking cell wall, cell produce
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