New Fixed Laccase Complex Vector Synthesis And Degradation Of Dye

The wastewater from dye and printing industries, leading to serious pollution, is one of the industrial wastewater which is the most difficult to treat. Many scholars focus on the study of immobilized enzyme on treating the dye wastewater. Immobilized laccase has special enzyme degradation mechanism and extensive substrate specificity; besides, compared to free laccase, it is not easy to inactivate and is able to reuse, so immobilized enzyme has the unordinary advantage in the degradation of the dye and wastewater, showing wide application prosperity. The purpose of this thesis is to synthesize a kind of composite carrier to immobilize laccase, to investigate degradation effect of immobilized laccase on Acid Violet 43, to analyze the degradation product by means of GC-MS, and to give the possible way of laccase's degradation on Acid Violet 43.A new kind of acrylic copolymer with composite acticarbon was prepared with the suspension polymerization. The group monomers were GMA (glycidyl methacrylate) and MMA (metercrylic acid methanoate), the content of which were 40% and 30% of the total monomer quantity, respectively. The crosslinking agent was DVB (ortho-divinylbenzene), 30% of the total monomer quantity. The content of additive agent, acticarbon, was 15% of the total monomer quantity. The aqueous phase was the CaCl₂ solution, and the volume ratio of aqueous phase to oil was 3.5:1. The copolymer prepared contained epoxide group, with the content of surface epoxide group 1.8mmol/g, and porous surface, and its structure makes it suitable for the immobilization of enzyme.From a host of experiments, it can be concluded that the optimum conditions for immobilization were: the laccase quantity 100 u/ ml, immobilization temperature 30℃, immobilization time 24 h, pH6.5. After the immobilization, the thermal stability and the pH stability both obviously improved compared to the free laccase, the optimum pH was increased, and the proper pH range was enlarged.After preparing the immobilized laccase, its degradation on Acid violet 43 was investigated. Considering both optimum temperature and thermal stability of immobilized laccase, 50℃ was the optimum temperature. With the increase of immobilized laccase amount, the decolor rate of Acid Violet 43 enlarged. It was determined that the immobilized laccase amount was 0.5g (wet weight), with the optimum pH 5.0, and reaction temperature 8 h, which the decolor rate reached 98.6%; the immobilized laccase can be reused 14 times, whose activity only lost 14.7% after incubating for 1h at 70℃. Through investigating the effect of different materials on the decolor rate of Acid Violet 43 and the different absorption spectrum of the Acid Violet 43 between the pre-reaction and post-reaction, it was concluded that the decolorization of Acid Violet 43 with the immobilized...