Micron Scale Covalent Bonding Type Epoxy Carrier Of The Preparation, Characteirstic And The Immobilized Enzyme

Enzyme catalysis has efficiency, green, low carbon, sustainable and unique advantages.Compared with the free enzymes, immobilized enzyme fixed in insoluble carriers through thephysical or chemical method have the advantage of recovery, repeated use, and increasedstability, so they significantly reduce the economy of the biological catalysis process.Immobilized enzyme in food processing, enzyme sensor, biological chemical catalytic and chiraldrugs split of enzymatic field has good application prospect. Carrier and enzyme covalentbinding is one of the important ways to get immobilized enzyme. In traditional covalent bondingcarrier before using need tedious activation of the program, enzyme molecule covalent bondingneed severe immobilized conditions, immobilized enzyme of low energy recovery, poorstability, and unit of enzyme quantity quality carrier fixed lower severely restricts covalentbinding of carrier in the catalysis industrial application, the aim of this paper is to developcarriers which can covalent bonding with enzyme molecule in the warm conditions, with highefficiency (enzyme loading capacity, high energy recovery, operating stability), and withoutactivation before using.Use emulsion polymerization and suspension polymerization synthesisactive carrier, and The characterization of the synthesis active carrier was conducted by scanningelectron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), magnetic vibration(VSM).Research the influence of synthesis conditions on the carrier morphology, size, apertureand other chemical factors, this paper discussed the chemical modification techniques, evaluatedthe immobilized enzyme efficiency.The suspension polymerization technology of methyl methacrylate shrink glyceride(GMA), methyl methacrylate-2-hydroxyl ethyl ester (HEMA), glycol two methylacrylic acidester (EGDMA) as polymerization monomer, benzoyl peroxidet as trigger reagent, and theprepared epoxy base Poly (GMA-EGDMA-HEMA) carrier is single scattered, surface smooth,approximately spherical, its size is about200μm, surface area of87.34m~2/g, aperture is0.11ml/g. Through the ethylenediamine chemical modification, epoxy carrier Poly(GMA-EGDMA-HEMA) part of the surface of the active epoxy turned into amino. Withoutsurfactants, methyl methacrylate shrink glyceride (GMA), methyl methacrylate-2-hydroxyl ethylester (HEMA), glycol two methylacrylic acid ester (EGDMA) as polymerization monomer,ammonium persulfate as the trigger reagent, with polyethylene alcohol concentration asstabilizer,the prepared magnetic epoxy Poly (GMA-EGDMA-HEMA) carrier, approximatelyspherical, average particle size of about400nm, specific surface area622.65m~2/g. The biggestcarrier magnetic saturation value of60.2emu/cm3, carriers through the magnetic field is easy to separate and recycling. The initial enzyme concentration of3.0mg/ml, pH6.5, the concentrationof the ions of0.5mol/L, pH7.0phosphate buffer solution system, epoxy Poly(GMA-EGDMA-HEMA) carrier on k.f ragilis β-D-galactosidase immobilization quantity is145.6mg/g, activity recovery of immobilized enzyme is72.6%. Immobilized enzyme has goodoperating stability, after partial continuous reaction of10times, the activity remaining for81.5%., immobilized k. fragilisβ-D-galactosidase catalytic synthesis of galactooligosaccharidesthe optimum temperature is30℃, and reaction4hours the galactooligosaccharides maximumyield.