Acrylamide Induced Mouse Cells, Dna Damage And Antioxidant Protective Effect Study

Objective This study was to detect the DNA damage and repair of various cells of mice treated with acrylamide(AA) by using comet assay, so as to investigate the genotoxicity of AA further, compare the sensitivity of different cells to AA and determine its possible genotoxic target organs.After being administered certain dose of AA,vitamin C or lycopene+AA, The DNA damage of peripheral lymphocytes and liver cells and the degree of lipid peroxidation,the activity of antioxidase of mice were also detected. It can reveal the correlation between genetoxic of AA and lipid peroxidation, and provide some experimental evidence for the mechanisms and prevention of genetoxic of AA.Method 1.In the study of time-effect relationship, DNA damage in the cells of peripheral lymphocytes, bone marrow, liver, lung, spleen, kidney and testicle of mice were detected by using comet assay at 0,3,6,12,24h after 50mg/kg AA intraperitoneal injection. And in the study of dose-effect relationship, DNA damage were detected at 12h after 0,10,25,50mg/kg AA intraperitoneal injection. 2. In order to study the effect of vitamin C and lycopene on the toxicity of AA, mice were administered 50mg/kg vitamin C or 10mg/kg lycopene for successive 10 days, and 50mg/kg AA from 6th day. The DNA damage of the peripheral lymphocytes and liver cells and the content of malondialdehyde(MDA),the activity of superoxide dismutase (SOD) were detected.Result 1.Significant increase in comet tail length,tail DNA% and tail moment(TM)were indu- ced at 3h in the liver and spleen cells and at 6h in bone marrow cells and peripheral lymphocyt- es after intraperitoneal treatment of AA at a dose of 50mg/kg(P<0.05).The indexes achieved maxlmum at 12h(P<0.01)and decreased at 24h,but were still significantly higher than the control (except spleen). Significant DNA damage of testicle cells was induced at 12h(P<0.01). No obvious increase in DNA damage was observed in the lung and kidney cells(P>0.05). 2. compared with the control group,the comet indexes showed significant increase in bone marrow cells in the 10mg/kg group,while in peripheral lymphocytes,liver and testicle cells,they increased in the 25mg/kg group(P<0.05).3. Treatment of AA for 5 days resulted in a significant increase in the content of MDA and decrease in the activity of SOD in serum and liver of mice(P<0.05). Compare with the AA group,The comet tail length,tail DNA% and TM of liver and peripheral lymphoc- ytes obviously decreased in the mice administered vitamin C or lycopene(P<0.05),the content of MDA decreased and the activity of SOD increased significantly(P<0.05).Conclusion 1. AA could induce DNA damage of peripheral lymphocytes, liver, bone marrow, testicle and spleen cells in mice. The DNA damage caused by AA could be repaired to a certain degree. 2. There are differences in sensitivity to acry1amide and the capability of DNA damage repaire of various cells in the mice. 3. It is suggested that the comet tail of peripheral lymphocytes is suitable as a biomarker for reflecting the DNA damage of organs after treatment of AA. 4. The DNA damage caused by AA is relevant with the lipid peroxidation in mice. 5. Vitamin C and lycopene could protect against the lipid peroxidation in mice treated with AA, and significantly decrease the levels of DNA damage in mice.