| As a carotenoids of immense potency, astaxanthin can not only eliminate the free radical. Resist lipidperoxidation, but also be anti-cancer, anti-aging, improve immunity. In recent years, The study of astaxanthin antioxidant effect is deepened. Acrylamide (Acrylamide, ACR) is one of the common chemical raw materials, and has been widely used in industry and research. ACR is a cumulative neurotoxic substance, reports indicated that the mechanism of nerve injury may be due to its role of oxidative damage to the nervous system. Therefore, this subject SD neonatal rat hippocampal nerve cell damage from acrylamide, explore the protective effect of astaxanthin hippocampal neurons. Astaxanthin in order to study the protective effect of hippocampal nerve cell damage caused by acrylamide, the experiments were the protective effect of the three aspects of the viability of hippocampal neurons, antioxidant enzyme systems and mitochondrial damage caused by acrylamide from astaxanthin expand the research.Primary Isolation and culture experiments first SD neonatal rat hippocampal neurons, and neuron-specific enolase antibody (NSE) staining of primary cultured hippocampal neurons were identified. To study the protective effect of astaxanthin on the viability of hippocampal neurons injury, the experiment by MTT colorimetric detection of cell viability, morphology of the nerve cells in the hippocampus was observed using an inverted microscope; using colorimetry hippocampus the nerve cells in the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity were measured astaxanthin hippocampal nerve caused by acrylamide cell antioxidant enzyme systems Injury; hippocampal nerve cells mitochondrial activity by MTT assay, chemical colorimetric determination of malondialdehyde hippocampus nerve cell mitochondria MDA content of nitric oxide NO astaxanthin acrylamide due to the protective effect of mitochondrial damage in hippocampal neurons.NSE immunohistochemical identification results show that the the cell cell body brown positive staining, primary cultured hippocampal neurons cultured cells; After acrylamide injury, the survival of nerve cells in the hippocampus was significantly decreased (P<0.01), while shrimp The astaxanthin is high, the middle dose group (1.0x10-5,1.0x10-6mol·L-1) can significantly improve the survival of nerve cells in the hippocampus (P<0.01). Inverted microscope, acrylamide hippocampal nerve cells can not adherent growth, morphology by a serious injury, astaxanthin high dose group (1.0x10-5mol·L-1) the majority of hippocampal neurons restored adherent growth, indicating that the astaxanthin due to acrylamide the hippocampal nerve morphology injury has a protective effect; the acrylamide group hippocampal nerve cell activity of SOD, CAT and GSH-Px activity was significantly lower (P<0.01), while astaxanthin, middle dose group (1.0x10-5,1.0x10-6mol·L-1) can significantly improve the activity of SOD, CAT activity and GSH-Px activity (P<0.01); the acrylamide group hippocampal neurons mitochondrial activity was significantly lower (P<0.01), astaxanthin low-dose group (1.0x10-7mol·L-1) to improve mitochondrial activity (P<0.05), astaxanthin, middle dose group (1.0x10-5,1.0x10-6mol·L-1) can significantly improve mitochondrial activity (P<0.01). Acrylamide hippocampal nerve cells mitochondria MDA, the content of NO was significantly higher (P<0.01), astaxanthin, middle dose group (1.0x10-5,1.0x10-6mol·L-1) of MDA, NO content lower (P<0.01). Therefore, astaxanthin can inhibit the viability of hippocampal neurons decreased; damage to the hippocampal nerve cells caused astaxanthin acrylamide antioxidant enzyme system has a protective effect of acrylamide; astaxanthin acrylamide The role of hippocampal neurons mitochondrial damage has a protective effect.By the conclusions of this study to draw the following aspects:astaxanthin hippocampal nerve cell damage caused by acrylamide has a protective effect and its mechanism is closely related to its antioxidant activity. |
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