The Black Lines Of Different Geographical Populations Of Hamster Fsh¦Â Partial Sequences Of Clones And Snp Analysis

As special functional groups, rodents plays an important role in the maintenance and balance of the ecosystem. However, too large quantity will cause grat harm to our production and livelihood. Cricetulus barabensis distributed widely and as one of the dominant species of injuricus rodents at North of China has grest capabities of reproduction. As the particularly harmful to crops, cricetulus barabensis has attracted grawing attention.Follicle stimulating hormone also known as FSH, which was a glycoprotein hormone composed by basophilic cells in the anterior pituitary, it has the ability to promote maturation and maintain breeding. In this paper, we chose Cricetulus barabensis from Yinan, Linqu and Qufu, there 2646bp of FSHβgene was cloned using PCR technique. The primers were designed according to the homologous species from GenBank, This part of FSHβgene was then cloned and sequenced in each more than 30 individuals in the three geographical populations.Then 33 SNPs were examined, including 2indels Insert/Deletion,49 haplotypes were defined in 91 samples. The sequence numbers submitted to the GenBank were HM063428,HM099995-HM100042. The mRNA sequence were compared with the corresponding sequence of the other 20 species embodied in GenBank. It showed high identities in the level of both nucleotide acids(78%～100%)and amino acids(75%～100%). The number of haplotye(H), haplotype diversity(Hd), nucleotide diversity(π), number of polymorphic sites(S), theta from S(θw) and average number of nucleotide differences (K) of FSH(3 gene of the three geographical populations were calculated with DnaSP5 software, which showed that the genetic diversity arrayed as Yinan, Qufu and Linqu.In addition, Fst, Nm and Dxy proved the genetic differentiation among the three populations existed in a low level. The result of Gst analysis among three populations arrayed from 0.015 to 0.006, which meant that 98.5%～99.4% mutation existed in each population while 1%～5% mutation existed among populations.ME analysis showed that 91 samples intersected with each other instead of forming three different groups consistent with geographic differentiation, which demonstrated the genetic differentiation among the three populations was not distinct. The research showed that FSHβgene on C. barabensis from Yinan, Linqu and Qufu was rich in haplotype diversity and nucleotide diversity.