Self-assembly Protease Microparticles For Liquid Detergent

Liquid detergent become a developing goal in the detergent industry, as it possesses the properties of environmental protection, mild, and easy to realize multi-function washing. However, due to the unstable of enzyme molecules in the liquid detergent, liquid washing detergency was generally low. Therefore, the issue of stable becomes the bottleneck in the developing of liquid detergent.In order to solve this difficult problem, this paper selected the biocompatible non-ionic surfactant-PEG-PPG-PEG and cyclodextrins (CD) as raw materials. CD and PPG will form inclusion because of Host-Guest recognition, then the hollow spheres were prepared by self-assembly. When the alkaline protease was in the aqueous solution, the alkaline protease can be encapsulated in the hollow spheres efficiently (the encapsulation efficiency can reach 89.54%). At the same time, the enzyme activity won’t be affected. The results show that:the PEG-PPG-PEG/CD/alkaline protease particles were stable in the liquid storage environment. The particles were collapsed and released the alkaline protease when the particles were in the washing environment. The optimum pH of encapsulated enzyme was 8 and the optimum temperature was 40℃. Under different pH and temperature condition, the stability of encapsulated enzyme was all higher than native enzyme. For example, under 25℃ and pH 9 conditions, the relative enzyme activity of encapsulated enzyme was almost double higher than the native enzyme and the native enzyme stored in the traditional stabilizers system when the samples were stored for eight days. At the same time, in the liquid detergent environment, the stability of encapsulated enzyme was not only higher than the native enzyme, but also higher than the current best enzyme stabilizer system.This method can parcel the protease and protect the enzyme molecules by one step self-assembly in order to maintain the stability of the protease in the liquid detergent. While in the washing environment the particles were collapsed and released the enzyme molecules to express its utility. This method provides new method and idea for solving the stability of enzyme in the liquid detergent.