The Hypoglycemic Effect Of Phellinus Linteus Mycelium Extract And Chitooligosaccharides

In previous studies, Phellinus lintues and other natural substance have been shown to possess significant hypoglycemic effect. However, many limiting factors of natural resource development greatly restrict and application of natural hypoglycemic ingredients. In this study, Phellinus lintues mycelia extract(EPLS) obtained by solid fermentation and chitooligosaccharides(COS) obtained by enzymatic hydrolysis were used as research objects, and their hypoglycemic effect on alloxan-induced type I diabetic mice were focused on. The results are as follows:1. The different doses of EPLS(polysaccharide content 54%) were orally administered into diabetic mice for 4 weeks. After 3 weeks, a significant reduction in water intake between model group and each dose group was appeared(p<0.05). After 4 weeks, EPLS at the middle and high doses could decrease fasting blood glucose values of diabetic mice 34.22% and 12.66%, respectively. Compared with the model group, the Hb A1 c of high dose group was significantly decreased(p<0.05) and the body weights of each dose group were significantly increased. The result of oral glucose tolerance test(OGTT) showed that blood glucose values of each dose group at 0.5h and 2h were significantly lower than that of model group(p<0.05). It was also found that EPLS played significant roles in promoting thymus development at the low and middle doses. Moreover, the mortality of mice in each dose group reduced 11.67%, 16.67% and 41.67%, respectively, in comparison with the model group. In summary, EPLS could effectively regulate blood glucose levels of type I diabetic mice and improve their symptoms of hyperglycemia.2. Different doses of COS(Mw = 1550) were used for animal oral test. After 2 weeks, a significant reduction(p<0.05) appeared between experimental groups and model group in water intake, and the food intake at low dose group also had a significant decrease(p<0.05). After 4 weeks, the fasting blood glucose level at high dose group decreased 15.05%. The results of OGTT showed that the blood glucose levels at 0.5h and 2h were significantly lower than that of model group(p<0.05). Thymus and spleen index of mice in each dose group were significantly increased(p<0.05) after the test. Low dose COS also caused the reducing of serum MDA content significantly(p<0.05). The mortality of mice in each dose group compared to model group reduced more than 12.82%. In summary, COS could effectively improve symptoms of hyperglycemia in type I diabetes mice.3. On the basis of above studies, EPLS and COS were combined to administer orally, then we test the fasting blood glucose, body weight, water intake, food intake, glucose tolerance, Hb A1 c, antioxidant and immune status and other indicators. The results showed that the two combined had a positive hypoglycemic effect. By using Webb’s fraction multiplication law to analysis the data, it was found that the two combined showed a synergy in regulating the blood glucose, reducing water intake, regulating serum SOD and Hb A1 c content under partial dose. Compared to the hypoglycemic effect of individual components, the synergistic effect is not obvious, experimental expectations were not reached.