Preparation Of Boronate Affinity Adsorbent Via Surface-initiated Reversible Addition Fragmentation Chain Transfer Polymerization And Their Application In Isolation And Enrichment Of Nucleosides

Cis-diol-containing biological molecule is a kind of important compounds widely distributed in nature, which include, but not limited to carbohydrates, glycoproteins, RNA, nucleosides and some flavonoids compounds and polyphenols. However, it is very hard to directly detect these substances due to their trace levels and the complex matrix. Therefore, high efficient isolation and enrichment of the cis-diol containing biomolecules are very critical for their analysis. The boronate affinity materials have higher selectivity towards cis-diols, and have been widely used for isolation and enrichment of biomolecules that containing cis-diols.In this study, we synthesized a new boronate affinity adsorbent by surface initiated reversible addition fragmentation chain transfer polymerization (SI-RAFT) based on commercial aminated poly(glycidyl methacrylate) (PGMA) microspheres, and applied the adsorbent for the specific capture of the nucleosides from calf serum. The main content including:(1) Firstly, commercial aminated poly(glycidyl methacrylate) (PGMA) microspheres were modified with chain transfer agent (CTA), S-1-dodecyl-S-(a, a-dimethyl-a-aceticacid)trithiocarbonate (DDATC). Then boronate affinity adsorbents were prepared via SI-RAFT polymerization employing 3-acrylamidophenylboronic acid (AAPBA) as the monomer. The prepared boronate affinity adsorbents possess much higher adsorption capacity, faster adsorption and desorption speed towards nucleosidesand higher selectivity to nucleosides; it possesses a maximum adsorption capacity of 99.2 μmol/L to adenosine, and the adsorption can reach equilibrium with 2 min.(2) The new boronate affinity adsorbents were employed for sample pretreatment before HPLC-UV analysis of nucleosides in calf serum. The effect of the binding pH, salt concentration and the amount of adsorbent on the extraction efficiency of nucleosides was investigated to oprimize the adsorption experiments conditions. The results indicated that the nucleosides can have highest extraction efficiency when the binding pH was 8.5-9, the amount of adsorbent was 100 mg. Under the optimal extraction conditions, the concentrations of uridine and guanosine in the calf serum were found respectively to be 170±11.6 ng/mL and 39.6±4.4 ng/mL by the standard addition method.