Effect Of Irradiation Pretreatment On Physicochemical Properties And Fermentation To Produce Alcohol Of Corn Flour

Corn is the world’s total output of the highest food crops, is the main raw materials of industrial alcohol and liquor. Due to the nature of cornmeal itself, there are some difficulties in the raw material of liquefaction and saccharification process, but irradiation technology can change the physical and chemical properties of corn flour, make it easier to apply in alcohol fermentation industry.This paper is the use of different irradiation dose processing corn flour, in-depth study the influence of the irradiation for corn flour particle morphology, physical and chemical properties, alcohol fermentation, the purpose is for the future application of irradiation technology in alcohol fermentation industry to provide the reference and theoretical basis. This study for the irradiation technology application in the field of development and the development of alcoholic fermentation industry has very important practical significance. The specific research contents and results are as follows:(1) Not irradiation corn flour and irradiation treatment on the particle properties of corn flour are respectively studied by using scanning electron microscope. The results showed that after irradiation treatment of corn flour particle surface appear the phenomenon on cave hole and be broken.(2) The irradiation dose of 10 ~ 100 Mrad corn flour has carried on the research of the physical and chemical properties. The results show that with the increase of irradiation dose, the surface of the corn flour color darker. The total sugar content of corn flour decrease, and the amount of reducing sugar and oligosaccharide increased; The acidity of corn flour gradually increased, but light transmittance, the average degree of polymerization and chemical stability is gradually reduced; The solubility of corn flour increased, the swelling degree is reduced, and as the change of irradiation dose presents the regularity of change of gradient.(3) Selection of irradiation dose of 50 Mrad corn flour is for enzymatic hydrolysis sugar experiment. The results showed that the optimum liquefaction condition for: the liquefaction temperature is 90℃, the liquefaction time is 70 min, the liquefaction p H value is 6.4, the amount of liquefied enzyme was 0.8 mg/g; the optimum saccharification condition for: the saccharification temperature is 70℃, the saccharification time is 90 min, the saccharifying p H value is 4.6, the amount of saccharifying enzyme is 2.4 mg/g, thereducing sugar yield of enzymatic hydrolysis of irradiation corn flour is increased than not irradiation by 47.74%.(4) The selection of irradiation dose of 50 Mrad corn flour carries out clinker alcohol fermentation experiments. The results show that the optimum fermentation conditions for: the ratio of water to material is 2.5:1, the amount of liquefied enzyme is 0.6 mg/g, the liquefaction time is 80 min, the amount of saccharifying enzyme is 2 mg/g, the saccharification time is 40 min, the amount of inoculation is 5%, the ethanol production of clinker fermentation of irradiation corn flour is increased than not irradiation by 20.41%.(5) The irradiation dose of 50 Mrad corn flour is selected for alcohol uncooked material fermentation experiments. The results show that the optimum fermentation conditions for: the ratio of water to material is 3:1, the amount of liquefied enzyme is 0.6 mg/g, the amount of saccharifying enzyme is 2.8 mg/g, the amount of cellulose enzyme is 0.15 mg/g, the amount of inoculation is 5%, the ethanol production of uncooked material fermentation of irradiation corn flour is increased than not irradiation by 5.18%.