Analysis Of Fatty Acids Of Intramuscular And Expression Of Related Genes Changes During The Growth Of Ujumqin Sheep

To investigate the relationship between the fatty acid contents, cholesterol contents and gene expressions in skeletal muscle of Ujumqin sheep, two major muscles including semitendinosus and longgissimus dorsi was obtained from different months. The contents of fatty acid and cholesterol were measured by Gas chromatography and colorimetric determination, respectively. And, the gene expressions of H-FABP, DGAT1, PPARα and LPL in skeletal muscles were analyzed by real-time PCR. The results showed that 31 fatty acids were identified in skeletal muscle of Ujumqin sheep. The saturated fatty acids (SFA) were increased from 1 to 6 months. No significant difference (P>0.05) of SFA were observed between 6-month-aged and 9-month-aged of Ujumqin sheep, the same to between 9-month-aged and 12-month-aged in skeletal muscles of Ujumqin sheep. Compared with 6 months-aged, the content of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) at 9 months-aged and 12 months-aged in skeletal muscles were decreased.18 months-aged were significantly higher than other months (P <0.05). The content of cholesterol were decreased from 1 to 6 months, afterwards increasing at 9 month. Compared with other months, the content of cholesterol at 18-month-aged were decreased in semitendinosus of Ujumqin sheep. The content of cholesterol had no significant difference at 12 months-aged in skeletal muscle of Ujumqin sheep, while significant difference was observed in other months-aged skeletal muscle (P<0.05). The expression level of H-FABP, DGAT1, PPAR a and LPL genes were not significantly different between the different months-aged skeletal muscle, while significant difference was observed between the various parts in skeletal muscles of Ujumqin sheep (P<0.05). The expression level of H-FABP at first 6 months-aged was low, at the same time, the expression level of H-FABP was higher from 9 to 12 months, and relatively, the 18 months-aged to achieve the highest. The expression level of DGAT1 was the highest at age of 18 months, and 6 months was in the second place in semitendinosus muscle of Ujumqin sheep. The expression level of DGAT1 was the higest at age of 6 months, and 18 months was in the second place in longgissimus dorsi muscle of Ujumqin sheep. The expression level of PPAR a was increased from 1 to 18 months of age, the expression level of PPAR a at 1 months-aged achieve the lowest level in semitendinosus muscle of Ujumqin sheep, and the same to 12 months-aged in longgissimus dorsi muscle of Ujumqin sheep. The expression level of LPL were increased from 1 to 3 months of age, and 6 months-aged were decreased compared with the others months, while the 18 months-aged achieved the highest. There was a positive correlation between the contents of saturated fatty acid, H-FABP and PPAR a gene expression levels within M. semitendinosus of Ujumqin sheep (P<0.01, P<0.05, respectively). And, the another positive correlations were observed between PPARα gene expression level, the contents of saturated fatty acid (P<0.01), polyunsaturated fatty acid, n-6 fatty acids, n-3 fatty acids (P<0.05).H-FABP、DGAT1、PPAR α can be used as a candidate gene regulation of fatty acid deposition. To find out relationship between the fatty acid contents and gene expression levels, which would be a potential theory for processing of animal products and muscle mass, fatty acid contents, cholesterol contents and gene expressions in skeletal muscles of Ujumqin sheep in different ages were invetigated in this study.