Adsorption Of Bsa On Regenerated Sugarcane Bagasse Cellulose Microspheres

In this paper,1-Allyl-3-methylimidazolium chloride ([Amim]C1) ionic liquid and Sugarcane baggage cellulose were adopted to prepare the regenerated cellulose microspheres by reversed phase suspension polymerization technique. With the adsorption capacity of BSA as an index, the preparation precedures of cellulose microspheres were optimized. The effects of adsorption time, pH, temperature, concentrations of albumin bovine on the adsorption of bovine serum albumin were studied. The thermodynamics parameters in adsorption process were calculated, the mechanism of adsorption was preliminarily discussed.First, cellulose were extracted from the Sugarcane bagasse by acid-alkali method, allyl chloride and N-methylimidazolium were adopted to prepare the [Amim]Cl ionic liquid. Suger bagasse cellulose and ionic liquid were investi-gated, the component concentration of suger bagasse cellulose were analysed by Chinese standard, the structure of [Amim]Cl ionic liquid were investigated by means of 1HNMR and FTIR, and the dissolving of cellulose in [Amim]C1 ionic liquid were studied.Second, cellulose/[Amim]Cl solution were adopted to prepare the cellulose microspheres by reversed phase suspension polymerization technique. The preparing conditions of the cellulose microspheres by the reversed phase suspension polymerization technique were optimized by the orthogonal and signal factor experiments. The optimum operating conditions included 4.0% of the mass content of the cellulose, dissolution temperature was 80 ℃, liquid paraffin as continuous phase, cellulose/[Amim]Cl solution as dispersed phase 4:1 of volume ration of continuous phase and dispersed phase, Span80 as emulsifier, distilled water as curing agent,1:10 of volume ration of emulsifier and dispersed phase,2:1 volume ration of curing agent and dispersed phase,600 r·min-1 of the agitation speed, and the system temperature was 80℃, and the average diameter of the prepared cellulose microsphere was 10μm. under these conditions, the adsorption capacity of BSA was 82.6 mg·g-1.The physical properties of the prepared cellulose microspheres were studied by a series of measurgin methods such as optical microscope, GPC, FTIR, XRD, TGA and so on. The results showed that the cellulose microspheres were ivory particles, and the microsphere has round appearance, the polymerization degree of cellulose changed from 1273 to 138, the crystalline form transformed from cellulose Ⅰ to cellulose Ⅱ, the degree of crystallization changed from 54.58% to 35.31% after regenerated from [Amim]C1 ionic liquid. And the cellulose microspheres have excellent chemical resistance and thermal stability.In the end, Factors affecting adsorption capacity such as temperature, pH, initial BSA concentration and contact time were studied. Results showed that the maximum adsorption capacity of the phosphorylated cole straw was 116.67mg·g-1 at pH=4.9,20℃ and for 8 h. The experimental results indicated that the adsorption characteristic of cellulose microspheres for BSA adapted to langmuir adsorption isotherm equation, the adsorption thermodynamic of cellulose microspheres for BSA including enthalpy △H, free energy change △ G and entropy change △S are negative when temperature were in the range of 293.15 to 313.15K, which indicates the adsorption is an exothermic, spontaneous and entropy decreasing process, and the decrease of temperature would benefit the adsorption.