Supercritical Carbon Dioxide Technology Of Preparation Of Nanodrug Particles

Recently, due to the nano drug can effectively improve the difficult soluble drug dissolution and reducing the stimulation of gastrointestinal, and nano drug particles is widely applicated in the field of drug delivery. Hence, nano-drug technology has also been researched. In numerous methods of nano drug particles preparation, supercritical carbon dioxide as a new type of green technology is further used in indissolvable drug nanoparticles preparation. Compared with other traditional preparation technologies, nanoparticles prepared by supercritical carbon dioxide are less organic solvent residual and high operating controllability on particle diameter size and morphology. This paper chooses the non-steroidal anti-inflammatory drugs such as celecoxib and ketoprofen and ginseng saponin Rh2 as model drug, and researches the effect on the drug nanoparticles preparation by using supercritical carbon dioxide.First of all, with celecoxib and ketoprofen as a model drug, polymer Eudragit S100 as the carrier materials, this paper used Aerosol Solvent Extraction System (ASES) to prepare drug nanoparticles. This process is based on the particle size, morphology and drug loading, and inspected the influence principle of various factors to obtain the optimal process conditions. In this paper, the celecoxib particles with average size of about 40 nm and the ketoprofen particles with average size of about 50 nm have been prepared. The experimental results show that elevating system temperature and pressure and reducing the velocity and concentration of mixed solution are conductive to get smaller particles and narrower distribution of drug particles. As to the temperature effect on the drug loadings, different drugs have obvious differences. In addition, the results of FT-IR and XRD show that the chemical structures of the two drugs have no change after ASES process, but their crystal shape transformed from crystalline to amorphous type. Finally, compared with ingredients, nano drug particles showed obvious effect of pH response.Then, ginseng saponin Rh2 nanoparticles were prepared by Gas Antisolvent (GAS). The results show that with the increase of Poloxamer 188’s ratio, the particles’size decreased from 85-90 nm to 15-20 nm and particles were more evenly distributed. Besides, the results of FT-IR, XRD and 1HNR show that GAS process didn’t change the chemical structure of ginseng saponin Rh2, and crystal shape transferred from crystalline to amorphous type. Dissolution results showed that the dissolution rate of particles increased 4 times compared with the ingredient. Cytotoxicity experimental results show that ginseng saponin Rh2 nanoparticles have obvious inhibitory effect on Hela cell and concentration dependence.