| As an important genetic material, the inverse parallel double helix model of DNA opened up the field of molecular biology to a new page. Apart from its role as the genetic material, however, DNA have great potential in the field of nanometer material science and targeted therapy with its special chemical and biological characteristics. With the further development of research on DNA for several years, DNA nanotechnology is used to construct 2D or 3D nanostructures as an emerging nanotechnology, showing a wonderful and colorful world for human. In the assembly of nano scale, the selection of construct the binding site motif is very important. According to the design ideas of experiment, the "close to" or "far from" assembly structure has a great influence on the optical properties. Therefore, it is a difficult problem to accurately control the assembly binding site to get a photoelectric signal in the research of assembly in DNA. The appearance of DNA origami provides an effective solution to this challenge. DNA Origami is a unique method to assemble. According to the pre designed structures, the long chain and short chains were mixed together. Then short chains make long chain folded into a desired structure just like staples. This novel method is advantaged, which can be pre designed site, good biocompatibility and can be assembled into material diversity Therefore, DNA Origami has become a new hotspot in the research, because it can be designed to the precise regulatory sites, stoichiometric and the structure of the product is easy to be purified. The assembly of noble metal nanoparticles and DNA Origami can be accurately designed in regulatory sites, in order to control the optical signal intensity better. The assembly of noble metal nanoparticles and origami will lead to a stronger surface plasmon resonance phenomenon, which is called the surface enhanced Raman scattering (SERS).All along, the assembly dimension guided by DNA is small, mostly in nano size, and even less research in mesoscopic and macro field. However, the properties of accurate DNA base pairing are lack in a lot of supraniolecular materials. So it is necessary to extend the self-assembly guided by DNA to the field of mesoscopic.This thesis is mainly divided into three parts:1. Introduce the research and application of the classic model of DNA Origami in the field of biology nano medicine;2. The noble metal nanoparticles (gold, silver) are assembled with DNA Origami, through the control of site to get the 3D assembly, introducing characteristic Raman molecule and surface enhanced Raman structure;3. The assembly of magnetic nanoparticles (MNPs) multilayer film on the surface of glass fiber, then the substrate and the glass fiber are modified with complementary DNA sequence. And lead glass fiber movement by the magnet, which is fixed on the surface of the substrate to get more complex design.In the second and third chapter of this paper, we designate that nanoparticles assembled to DNA folding structure through the unique DNA base pairing, achieving the accurate assembly of nanoparticles in the specified location. The surface enhanced Raman structure which can be controlled is got by introduce the characteristic Raman signal molecules. The Raman signal can be enhanced beyond 102 orders or even 103 orders of magnitude with the replacement of gold nanoparticles by silver nanoparticles among the assembly, which can be applied to the structure analysis and detection, etc. The fourth chapter of the paper, we introduce innovatively the assembly of glass fiber and quartz substrate guided by DNA. And we get more complex patterns in the operation of magnetic field, extending the assembly guided by DNA to the mesoscopic field, starting further application of the assembly guided by DNA to larger size... |
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