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Microbial Fermentation In The Production Corn Peptide Research

Posted on:2016-02-29Degree:MasterType:Thesis
Country:ChinaCandidate:J X MuFull Text:PDF
GTID:2191330473466799Subject:Bio-engineering
Abstract/Summary:PDF Full Text Request
Corn peptide is extracted from corn protein, through directional enzyme and specific small peptide separation of small molecular peptides. Have a lower blood pressure solution wine decanter corn peptide as a new type of health care products, health gradually in the market demand. Huge market prospects for the development of corn peptide.condition optimization; This topic through to yongchuan lobster sauce, corn processing plant near the moist soil, such as suitable for fermentation conditions were screened by certain conditions, by means of bacteria morphology, colony morphology and biochemical determination eventually determine the screening of strains of bacillus subtilis, and the strongest enzyme activity were screened by some experiments. Then the strains of victory conditions were optimized. By single factor experiment and orthogonal experiment after determined the optimal growth conditions of b. subtilis. Ultimately determine the optimal growth conditions of strain to 30 ℃, pH 7.8, inoculation fungus age for 10 h, bacteria growth advantage can fully display at this time. The concentration of bacteria growth is able to reach new heights. For later experiment of corn peptide provides excellent strains fermentation so as to ensure the smooth progress of the fermented corn peptide. e factor’s influence on corn peptide fermentation conversion, first is to bacillus subtilis of domestication, maize yellow powder medium through the domestication of the discovery, screening of bacillus subtilis can completely in the maize yellow powder medium domestication, and grew well. Fully demonstrated the strain can be corn peptide production and optimization. Through the experiment of fermentation time of corn peptide, ultimately determine the optimal fermentation period of corn peptide for around 85 h. Then of corn peptide in the process of fermentation temperature, table speed, inoculation quantity, the best ingredients than medium components such as comparison, the single factor experiment and through the response surface experiment, finally determined the optimum hydrolysis conditions of corn peptide. Through the experiment of fermentation time of corn peptide, ultimately determine the optimal fermentation period of corn peptide for around 85 h. Then of corn peptide in the process of fermentation temperature, table speed, inoculation quantity, the best ingredients than medium components such as comparison, the single factor experiment and through the response surface experiment, finally determined the optimum hydrolysis conditions of corn peptide. And under the condition of the fermented corn peptide, corn peptide may be the best yield reached 23%, more than 18% of the initial conversion rate is greatly improved. At the end of the project phase, and the physiological activity of corn peptide mainly oxidation resistance testing. Through the experiment discovered corn peptide oxidation resistance is obvious. And under certain conditions the oxidation resistance to oxidation resistance higher than vitamin c. It proved that corn peptide medicinal value.
Keywords/Search Tags:corn peptide, microbial fermentation, optimization conditions, oxidation resistance
PDF Full Text Request
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