The Optimization Of Corn Peptide Hydrolytics Processing And The Development Of Corn Peptide Functional Beverage

In recent years, many kinds of biological activity small peptides were already isolated from food protein hydrolysate, which with the activity of neural regulation, cellul arimmunity activation, cardiovascular function amendment and anti-aging. These small peptides contained less then ten amino acids, and can be absorbed in the intestine directly. The absorption rate was 2～3 times quickly compared to the free amino acid and protein, therefore the nutritional value and biology effect of bioactive peptides was higher than free amino acid and the protein. The corn protein is the corn starch producti onby-product. In our country, the corn protein was abundant, but it was utilized insufficiently and was discharged wastefully for its unbalanced amino acid composition and insolubility. This research using the corn protein as raw material, prepared anti-oxi dativepeptide via protease hydrolysis catalyzed by an alkaline protease. This research will notonly solve the corn protein processing problem, but also developed one kind of new function food. It was a beneficial exploration for extending the corn deep process ing industry chain and realizing high added value transformation of corn. The preparati on, purification, drying method, activity examination and function preparation develop ment ofcorn peptide have carried out in following research:1. Optimum condition of corn protein defatted using the sodium carbonate was determi ned, through the orthogonal experiment. the result show: sodium carbonate concentra tion is 40 g/L, the amount is 16 ml/g, the temperature is 40℃, time is10min, the defatted rate is 79.45%.2. Focused on optimization of its hydrolysis processing, examinated the antioxdation activity of hydrolysate. compared antioxdation activity under the different hydrolysis time, optimumal hydrolysis time is 4 hours, the degree of hydrolysis is 30%.3. Corn peptide spray drying processing parameter had determined through the orthogonal experiment: the result shows that the optimum processing parameter is inlet tem perature is 200℃, feed rate is 30ml/min, rotation velocity is 15000r/min.4. Ingredient of corn peptide was analyzed, the result is that in the corn peptide powder the peptide content is 72.31%, total sugar content is 18.45%, fat content is 0.37 moisture content is 4.1% ash contents is 4.8%.5. Dissolved corn peptide solid product 100mg in the 1mL water, on Sephadex the G15 column (16×1000mm, the eluent speed of flow is 1.0mLmin-1, the examination wave length is 220nm.) To separates the component to make the mass spectrum analysis, the corn peptide is the mixture, the molecular weight distributes in 200-1000D, mainly concentrates in 500-600D.6. Analyzed biological antioxdation activity system of the corn peptide, result shows: or n peptides have the significant antioxidant activity, the inhibition rate is 46.10%; it also has a strong radical scavenging activity in eliminating DPPH, OH, and super oxidation radicals.7. Determined experiment a corn peptide beverage the optimal formula through the orth ogonal is: The peptide content is 2%, the xylitol content is 8%, the apple acid content is 0.2%.8. Carried on the animal experiment anion to the corn peptide beverage, the experimental result indicated that, The corn peptide Corn-peptide beverage enforced the swimining durability of mice and The contents of the lactates and the plasma urea were lower, but the contents of liver glycogens and muscle glycogen were higher. Corn-peptide has anti-fatigue effect...